Satoh F, Takahashi K, Murakami O, Totsune K, Sone M, Ohneda M, Sasano H, Mouri T
The Second Department of Internal Medicine, Tohoku University School of Medicine, Sendai, Japan.
Neurosci Lett. 1996 Jan 26;203(3):207-10. doi: 10.1016/0304-3940(95)12294-x.
Adrenomedullin is a potent vasodilator peptide that was isolated from pheochromocytoma. Localization of adrenomedullin-like immunoreactivity was studied by immunocytochemistry in the human hypothalamus and adrenal gland. Adrenomedullin-immunoreactive cell bodies were found in the paraventricular, supraoptic and infundibular nuclei of the hypothalamus. Both magnocellular and parvocellular cells of the paraventricular nucleus were positively immunostained. Adrenomedullin-like immunoreactivity was localized in the adrenal medulla. No positive immunostaining was observed in the vascular endothelium, vascular smooth muscle cell or adrenal cortex. The preabsorption of the antiserum with synthetic human adrenomedullin (1-52) abolished the immunostaining. These findings indicate that adrenomedullin-like immunoreactivity is localized in the paraventricular, supraoptic and infundibular nuclei as well as in the adrenal medulla, and suggest that adrenomedullin acts as a neurotransmitter, a neuromodulator or a neurohormone in the human hypothalamus.
肾上腺髓质素是一种从嗜铬细胞瘤中分离出的强效血管舒张肽。采用免疫细胞化学方法研究了肾上腺髓质素样免疫反应性在人下丘脑和肾上腺中的定位。在下丘脑的室旁核、视上核和漏斗核中发现了肾上腺髓质素免疫反应性细胞体。室旁核的大细胞和小细胞均呈阳性免疫染色。肾上腺髓质素样免疫反应性定位于肾上腺髓质。在血管内皮、血管平滑肌细胞或肾上腺皮质中未观察到阳性免疫染色。用人合成肾上腺髓质素（1-52）对抗体进行预吸收可消除免疫染色。这些发现表明，肾上腺髓质素样免疫反应性定位于室旁核、视上核和漏斗核以及肾上腺髓质，并提示肾上腺髓质素在人下丘脑中作为神经递质、神经调质或神经激素发挥作用。
