通过彗星试验和微核试验评估紫菌素的遗传毒性的体外测试。
In vitro testing for genotoxicity of violacein assessed by Comet and Micronucleus assays.
作者信息
Andrighetti-Fröhner Carla R, Kratz Jadel M, Antonio Regina V, Creczynski-Pasa Tânia B, Barardi Célia R M, Simões Cláudia M O
机构信息
Laboratory of Applied Virology, Department of Pharmaceutical Sciences, Universidade Federal de Santa Catarina, UFSC, Campus Universitário Trindade, Florianópolis, SC, Brazil.
出版信息
Mutat Res. 2006 Jan 31;603(1):97-103. doi: 10.1016/j.mrgentox.2005.11.001. Epub 2005 Dec 13.
Chromobacterium violaceum is a Gram (-) bacteria found in water samples and soils from tropical and subtropical regions of the world. Violacein, the major pigment produced by these bacteria, has been shown to have antibiotic, antitumoral and trypanocidal activities. In the present work, the genotoxicity of violacein was investigated in four different cell lines by using the alkaline Comet assay and in VERO cells using the Micronucleus test. In the alkaline Comet assay, violacein, when tested at concentrations ranging from 0.19 to 1.5 microM, did not induce a significant increase in DNA damage in HEp-2 and MA104 cells. However, violacein was positive for DNA damage in FRhK-4 cells and for both DNA damage and micronuclei in VERO cells, in a concentration-response relationship. The results of this study indicated that violacein is genotoxic in VERO and FRhK-4 cells. These findings contribute to a comprehensive evaluation of the pharmacological potential of violacein.
紫色杆菌是一种革兰氏阴性菌，存在于世界热带和亚热带地区的水样和土壤中。这些细菌产生的主要色素——紫罗碱，已被证明具有抗生素、抗肿瘤和杀锥虫活性。在本研究中，通过碱性彗星试验在四种不同细胞系中以及在VERO细胞中使用微核试验研究了紫罗碱的遗传毒性。在碱性彗星试验中，当紫罗碱在0.19至1.5 microM的浓度范围内进行测试时，在HEp-2和MA104细胞中未诱导DNA损伤显著增加。然而，紫罗碱在FRhK-4细胞中对DNA损伤呈阳性，在VERO细胞中对DNA损伤和微核均呈阳性，呈浓度-反应关系。本研究结果表明，紫罗碱在VERO和FRhK-4细胞中具有遗传毒性。这些发现有助于对紫罗碱的药理潜力进行全面评估。
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