Department of Restorative Dentistry, Institute of Science and Technology, Univ. Estadual Paulista, Av. Eng. Francisco Jose Longo, 777, São José dos Campos, SP, 12245-000, Brazil.
Department of Cariology, Restorative Sciences and Endodontics, School of Dentistry, University of Michigan, Ann Arbor, MI, USA.
Clin Oral Investig. 2016 May;20(4):815-9. doi: 10.1007/s00784-015-1567-4. Epub 2015 Aug 29.
The development of endodontic sealers based on natural resins seems to be promising, given their improved biological properties. This study evaluated the cytotoxic and genotoxic effects of two experimental root canal sealers, based on extracts from Copaifera multijuga and Ricinus communis (castor oil polymer), comparing them to synthetic resin-based sealers: a single methacrylate-based, a multi-methacrylate-based, and an epoxy resin-based sealers.
Sealers were prepared, set, and exposed to cell culture medium for 24 h at 37 °C with CO2. V79 cells were exposed to serial dilutions of the extracts of each sealer for 24 h. Cell viability was measured by the MTT assay and genotoxicity was assessed by the formation of micronuclei.
The single methacrylate-based sealer had the most cytotoxic effects, with significant reduction in cell viability in all dilutions of the extract. The castor oil polymer-based sealer was, on the other hand, the most biocompatible sealer, with no cytotoxic effects at any concentration. All tested sealers were not genotoxic, excepting the single methacrylate-based sealer.
The tested natural resin-based sealers presented low cytotoxic and no genotoxic effects on cell cultures.
These results may suggest a good alternative to develop new endodontic sealers, in order to achieve better biological response and healing, when compared to commercially available sealers.
基于天然树脂的根管封闭剂的发展似乎很有前景，因为它们具有改善的生物特性。本研究评估了两种基于 Copaifera multijuga 和 Ricinus communis（蓖麻油聚合物）提取物的实验性根管封闭剂的细胞毒性和遗传毒性作用，将它们与基于合成树脂的封闭剂进行比较：一种单甲基丙烯酸酯基、一种多甲基丙烯酸酯基和一种环氧树脂基封闭剂。
将密封剂制备、设置并在 37°C 下与 CO2 暴露于细胞培养基中 24 小时。V79 细胞暴露于每种密封剂提取物的系列稀释液中 24 小时。通过 MTT 测定法测量细胞活力，通过形成微核评估遗传毒性。
单甲基丙烯酸酯基密封剂的细胞毒性作用最强，所有提取物稀释度均显著降低细胞活力。另一方面，蓖麻油聚合物基密封剂是最具生物相容性的密封剂，在任何浓度下均无细胞毒性作用。除了单甲基丙烯酸酯基密封剂外，所有测试的密封剂均无遗传毒性。
测试的天然树脂基密封剂对细胞培养物表现出低细胞毒性和无遗传毒性作用。
与市售密封剂相比，这些结果可能表明开发新的根管封闭剂的良好替代方案，以实现更好的生物反应和愈合。
