基于固定在复合生物聚合物膜中的酪氨酸酶的电化学生物传感器的研制。
Development of electrochemical biosensor based on tyrosinase immobilized in composite biopolymeric film.
作者信息
Tembe Sanket, Karve Meena, Inamdar Shaukat, Haram Santosh, Melo Jose, D'Souza Stanislaus F
机构信息
Department of Chemistry, University of Pune, Ganeshkhind, Pune 411 007, India.
出版信息
Anal Biochem. 2006 Feb 1;349(1):72-7. doi: 10.1016/j.ab.2005.11.016. Epub 2005 Nov 28.
An electrochemical enzyme electrode for dopa and dopamine was developed via an easy and effective immobilization method. The enzyme tyrosinase was extracted from a plant source Amorphophallus companulatus and immobilized in a novel composite of two biopolymers: agarose and guar gum. This composite matrix-containing enzyme forms a self-adhering layer on the active surface of glassy carbon electrode, making it a selective and sensitive phenol sensor. Dopa and dopamine were determined by the direct reduction of biocatalytically liberated quinone species at -0.18V versus Ag/AgCl (3M KCl). The analytical characteristics of this sensor, including linear range, lower detection limit, pH, and storage stability, are described. It has reusability up to 15 cycles and a shelf life of more than 2 months.
通过一种简便有效的固定化方法，研制出了一种用于检测多巴和多巴胺的电化学酶电极。酪氨酸酶是从植物魔芋中提取的，并固定在由琼脂糖和瓜尔豆胶这两种生物聚合物组成的新型复合材料中。这种含有酶的复合基质在玻碳电极的活性表面形成了一层自粘附层，使其成为一种选择性和灵敏的酚类传感器。通过在相对于Ag/AgCl（3M KCl）为 -0.18V的电位下直接还原生物催化释放的醌类物质来测定多巴和多巴胺。描述了该传感器的分析特性，包括线性范围、检测下限、pH值和储存稳定性。它具有高达15次循环的可重复使用性，保质期超过2个月。
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