School of Chemical and Biological Engineering, Seoul National University, Seoul, Republic of Korea.
Talanta. 2009 Dec 15;80(2):1007-11. doi: 10.1016/j.talanta.2009.08.032. Epub 2009 Aug 29.
A novel 3-dimensional single wall carbon nanotubes (SWNTs)-polypyrrole (Ppy) composite was prepared as an electrode by chemically polymerizing polypyrrole onto SWNTs using a LiClO(4) oxidant. This composite electrode was characterized by scanning electron microscopy (SEM) and cyclic voltammetry with 1 mM Fe(CN)(6)/Fe(CN)(6). The SWNTs were thickly coated with chemically polymerized polypyrrole and the composite had many surface pores and crevices which could enhance mass transfer. The SWNTs-Ppy composite electrode showed a large specific surface area (30 m(2)/g) and a good reproducible current response, at about 100 times the peak current of a glassy carbon electrode (GCE). The diffusion coefficient was calculated to be 4.81x10(-6) cm(2)/s. As a biosensor application, tyrosinase was immobilized on the functionalized SWNTs and tyrosinase-SWNTs-Ppy composite was prepared in the same manner. This tyrosinase-SWNT-Ppy composite electrode was used for amperometric detection of dopamine in the presence of ascorbic acid and showed high sensitivity (467 mA/M cm(2)) and lower detection limit (5 microM) compared to previous reports.
一种新型的三维单壁碳纳米管（SWNTs）-聚吡咯（Ppy）复合材料通过使用 LiClO(4) 氧化剂将聚吡咯化学聚合到 SWNTs 上来制备电极。该复合电极通过扫描电子显微镜（SEM）和 1 mM Fe(CN)(6)/Fe(CN)(6)的循环伏安法进行了表征。SWNTs 被厚厚的化学聚合聚吡咯包裹，并且复合材料具有许多表面孔和裂缝，这可以增强质量传递。SWNTs-Ppy 复合电极具有大的比表面积（30 m(2)/g）和良好的可重现电流响应，约为玻碳电极（GCE）的峰值电流的 100 倍。扩散系数计算为 4.81x10(-6) cm(2)/s。作为生物传感器的应用，将酪氨酸酶固定在功能化的 SWNTs 上，并以相同的方式制备酪氨酸酶-SWNTs-Ppy 复合材料。与以前的报道相比，这种酪氨酸酶-SWNT-Ppy 复合电极在存在抗坏血酸的情况下用于多巴胺的安培检测，具有高灵敏度（467 mA/M cm(2))和较低的检测限（5 microM)。
