Krueger Andreas, Hennings Ina C
Department of Molecular Parasitology, Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany.
Mol Phylogenet Evol. 2006 Apr;39(1):83-90. doi: 10.1016/j.ympev.2005.11.007. Epub 2005 Dec 15.
The molecular phylogenetics of the Simulium damnosum complex, including vectors of the human parasite Onchocerca volvulus, from various parts of Africa was studied and compared with results of cytogenetic analyses. The sequence data of mitochondrial 16s and nuclear ITS2 rDNA revealed that the complex comprises two main clades, roughly covering the more easterly and westerly African taxa, respectively. However, striking inconsistencies in the tree topologies turned up between the DNA fragments regarding the position of certain subcomplexes and species. The cytophylogenetic relationships are better reflected in the ITS2 tree where Simulium pandanophilum and Simulium mengense constitute a basal, Central African clade of the entire complex and are therefore suggested to be the chromosomal roots too. Further divisions and the corresponding biogeographic interpretations are discussed. Several species and cytoforms are placed within the system for the first time. The phylogenetic relationships within the complex do hardly correlate with host preferences or other behavioral and ecological characteristics.
对来自非洲各地的包括人类寄生虫旋盘尾丝虫病媒介在内的恶蚋复合体进行了分子系统发育研究，并与细胞遗传学分析结果进行了比较。线粒体16s和核ITS2 rDNA的序列数据显示，该复合体包括两个主要分支，分别大致覆盖非洲东部和西部的分类群。然而，在某些亚复合体和物种的位置上，DNA片段之间的树形拓扑结构出现了明显的不一致。细胞系统发育关系在ITS2树中得到了更好的体现，其中嗜潘丹蚋和孟氏蚋构成了整个复合体的一个基部的中非分支，因此也被认为是染色体根源。进一步的划分和相应的生物地理学解释也进行了讨论。首次将几个物种和细胞型纳入该系统。该复合体内的系统发育关系与宿主偏好或其他行为和生态特征几乎没有关联。
