Norice-Tra Carmelle T, Ribeiro José, Bennuru Sasisekhar, Fay Michael P, Tyagi Rahul, Mitreva Makedonka, Nutman Thomas B
Laboratory of Parasitic Diseases.
Laboratory of Malaria and Vector Research.
J Infect Dis. 2017 Sep 15;216(6):736-743. doi: 10.1093/infdis/jix394.
We have developed a serologically based immunophenotyping approach to study Onchocerca volvulus (Ov) population diversity. Using genomic sequence data and polymerase chain reaction-based genotyping, we identified nonsynonymous single-nucleotide polymorphisms (SNPs) in the genes of 16 major immunogenic Ov proteins: Ov-CHI-1/Ov-CHI-2, Ov16, Ov-FAR-1, Ov-CPI-1, Ov-B20, Ov-ASP-1, Ov-TMY-1, OvSOD1, OvGST1, Ov-CAL-1, M3/M4, Ov-RAL-1, Ov-RAL-2, Ov-ALT-1, Ov-FBA-1, and Ov-B8. We assessed the immunoreactivity of onchocerciasis patient sera (n = 152) from the Americas, West Africa, Central Africa, and East Africa against peptides derived from 10 of these proteins containing SNPs. Statistically significant variation in immunoreactivity among the regions was seen in SNP-containing peptides derived from 8 of 10 proteins tested: OVOC1192(1-15), OVOC9988(28-42), OVOC9225(320-334), OVOC7453(22-36), OVOC11517(14-28), OVOC3177(283-297), OVOC7911(594-608), and OVOC12628(174-188). Our data show that differences in immunoreactivity to variant antigenic peptides may be used to characterize Ov populations, thereby elucidating features of Ov population biology previously inaccessible because of the limited availability of parasite material.
我们开发了一种基于血清学的免疫分型方法来研究盘尾丝虫（Ov）种群的多样性。利用基因组序列数据和基于聚合酶链反应的基因分型，我们在16种主要的具有免疫原性的Ov蛋白基因中鉴定出非同义单核苷酸多态性（SNP）：Ov-CHI-1/Ov-CHI-2、Ov16、Ov-FAR-1、Ov-CPI-1、Ov-B20、Ov-ASP-1、Ov-TMY-1、OvSOD1、OvGST1、Ov-CAL-1、M3/M4、Ov-RAL-1、Ov-RAL-2、Ov-ALT-1、Ov-FBA-1和Ov-B8。我们评估了来自美洲、西非、中非和东非的盘尾丝虫病患者血清（n = 152）对源自这16种蛋白中10种含SNP蛋白的肽段的免疫反应性。在测试的10种蛋白中的8种含SNP肽段中观察到各地区之间免疫反应性存在统计学显著差异：OVOC1192(1-15)、OVOC9988(28-42)、OVOC9225(320-334)、OVOC7453(22-36)、OVOC11517(14-28)、OVOC3177(283-297)、OVOC7911(594-608)和OVOC12628(174-188)。我们的数据表明，对变异抗原肽的免疫反应性差异可用于表征Ov种群，从而阐明由于寄生虫材料有限而以前无法获得的Ov种群生物学特征。
