Burleson G R, Murray T M, Pollard M
Appl Microbiol. 1975 Mar;29(3):340-4. doi: 10.1128/am.29.3.340-344.1975.
Selected organisms with public health significance were placed in a reaction chamber for treatment by ozonation, by ozonation and sonication, by sonication, or by sonication during oxygenation. Vesicular stomatitis virus, encephalomyocarditis virus, GDVII virus, Staphylococcus aureus, Pseudomonas fluorescens, Salmonella typhimurium, enteropathogenic Escherichia coli, Vibrio cholerae, and Shigella flexneri were inactivated by treatment with ozone. When microorganisms were suspended in phosphate-buffered saline, they were inactivated rapidly by treatment with ozone. However, microorganisms suspended in secondary effluent from a wastewater treatment plant required longer contact times with ozone for complete inactivation. Simultaneous treatments by ozonation and sonication reduced the contact time for complete inactivation of microorganisms in secondary effluent. Treatment by sonication alone or sonication and oxygenation did not inactivate microorganisms. Therefore, the simultaneous treatment of microorganisms in secondary effluent with ozone and sonication resulted in a synergistic effect.
具有公共卫生意义的选定微生物被置于反应室中，分别采用臭氧处理、臭氧与超声联合处理、超声处理或充氧时超声处理。水泡性口炎病毒、脑心肌炎病毒、GDVII病毒、金黄色葡萄球菌、荧光假单胞菌、鼠伤寒沙门氏菌、肠道致病性大肠杆菌、霍乱弧菌和福氏志贺氏菌经臭氧处理后被灭活。当微生物悬浮于磷酸盐缓冲盐水中时，经臭氧处理可迅速灭活。然而，悬浮于污水处理厂二级出水的微生物需要与臭氧更长的接触时间才能完全灭活。臭氧与超声联合处理缩短了二级出水中微生物完全灭活所需的接触时间。单独超声处理或超声与充氧联合处理均不能使微生物灭活。因此，臭氧与超声联合处理二级出水中的微生物产生了协同效应。
