Kim Jung Gu, Ku Seung Yup, Lim Kyung Sil, Jee Byung Chul, Suh Chang Suk, Kim Seok Hyun, Choi Young Min, Moon Shin Yong
Department of Obstetrics and Gynecology, College of Medicine, Seoul National University, Seoul, Korea.
J Korean Med Sci. 2005 Dec;20(6):1017-22. doi: 10.3346/jkms.2005.20.6.1017.
The aims of this study were to investigate the relationships between the production of interleukin-1 (IL-1), and IL-6 system by whole blood cells, and bone mineral density (BMD), and polymorphisms in IL-1 system and IL-6 gene in postmenopausal Korean women. The production of IL-1alpha, IL-1beta, IL-1 receptor antagonist (IL-1ra), IL-6, and soluble IL-6 receptor (sIL-6r) by lipopolysaccharide-stimulated whole blood cells was measured by ELISA in 110 subjects. Serum osteocalcin, C-telopeptide of type I collagen, and BMD at lumbar spine and proximal femur were measured. IL-1alphaC(-889)T polymorphism, IL-1beta C(-511)T polymorphism, 86-base pair variable number tandem repeat polymorphism in the IL-1ra gene, and IL-6 C(-634)G polymorphism were analyzed. The production of IL-1beta correlated positively with BMD at femoral neck, whereas the production of other ILs did not correlate with BMD at the skeletal sites examined. No significant differences in the production of ILs were observed among normal, osteopenic and osteoporotic postmenopausal women, and among the different IL system polymorphisms groups studied. No correlation between bone turnover markers and the production of ILs was noted. In conclusion IL-1beta may regulate bone metabolism at femoral neck, and the IL system polymorphism do not affect the production of ILs by whole blood cells.
本研究旨在探讨绝经后韩国女性全血细胞白细胞介素-1（IL-1）和IL-6系统的产生与骨密度（BMD）之间的关系，以及IL-1系统和IL-6基因的多态性。通过酶联免疫吸附测定法（ELISA）检测了110名受试者脂多糖刺激的全血细胞产生的IL-1α、IL-1β、IL-1受体拮抗剂（IL-1ra）、IL-6和可溶性IL-6受体（sIL-6r）。测量了血清骨钙素、I型胶原C端肽以及腰椎和股骨近端的骨密度。分析了IL-1αC（-889）T多态性、IL-1βC（-511）T多态性、IL-1ra基因中86个碱基对可变数目串联重复多态性以及IL-6C（-634）G多态性。IL-1β的产生与股骨颈骨密度呈正相关，而其他白细胞介素的产生与所检测骨骼部位的骨密度无关。在正常、骨质减少和骨质疏松的绝经后女性之间以及所研究的不同IL系统多态性组之间，未观察到白细胞介素产生的显著差异。未发现骨转换标志物与白细胞介素产生之间存在相关性。总之，IL-1β可能调节股骨颈的骨代谢，且IL系统多态性不影响全血细胞产生白细胞介素。
