Kim Jung Gu, Roh Kyong Rock, Lee Jin Yong
Department of Obstetrics & Gynecology, Clinical Research Institute, College of Medicine, Seoul National University, Korea.
Am J Obstet Gynecol. 2002 Mar;186(3):345-50. doi: 10.1067/mob.2002.120483.
The objective of this study was to test the hypothesis that the cytosine-adenine polymorphism in the insulin-like growth factor-I gene is associated with serum insulin-like growth factor-I levels and bone mineral density.
The insulin-like growth factor-I cytosine-adenine polymorphism was analyzed in 300 postmenopausal Korean women. Serum insulin-like growth factor-I, bone alkaline phosphatase, and carboxy-terminal cross-linking telopeptide of type I collagen were measured by immunoradiometric assay and enzyme-linked immunosorbent assay. Bone mineral density at the lumbar spine and proximal femur was determined by dual energy radiograph absorptiometry.
Serum insulin-like growth factor-I and bone mineral density levels in women who were homozygous for a 194-base pair allele were significantly higher than those levels in the 194-base pair heterozygotes or women who did not possess the 194-base pair allele. A significantly decreased prevalence of the 194/194 genotype was observed in the combined group of women with osteopenia and osteoporosis, compared with normal women. No correlation between insulin-like growth factor-I genotypes and bone turnover markers was found.
The insulin-like growth factor-I gene cytosine-adenine polymorphism relates with circulating insulin-like growth factor-I levels and bone mineral density at the lumbar spine and proximal femur.
本研究的目的是检验胰岛素样生长因子-I基因中的胞嘧啶-腺嘌呤多态性与血清胰岛素样生长因子-I水平及骨密度相关这一假设。
对300名绝经后韩国女性的胰岛素样生长因子-I胞嘧啶-腺嘌呤多态性进行了分析。采用免疫放射分析和酶联免疫吸附测定法测量血清胰岛素样生长因子-I、骨碱性磷酸酶和I型胶原羧基末端交联肽。通过双能X线吸收法测定腰椎和股骨近端的骨密度。
纯合194碱基对等位基因的女性血清胰岛素样生长因子-I水平和骨密度显著高于194碱基对杂合子或不具有194碱基对等位基因的女性。与正常女性相比，骨质疏松症和骨质减少症女性联合组中194/194基因型的患病率显著降低。未发现胰岛素样生长因子-I基因型与骨转换标志物之间存在相关性。
胰岛素样生长因子-I基因胞嘧啶-腺嘌呤多态性与循环胰岛素样生长因子-I水平以及腰椎和股骨近端的骨密度相关。
