De Meutter J, Tytgat T, Prinsen E, Gheysen G, Van Onckelen H, Gheysen G
Vakgroep Moleculaire Genetica, Departement Plantensysteembiologie, Vlaams Interuniversitair Instituut voor Biotechnologie, Universiteit Gent, Belgium.
Commun Agric Appl Biol Sci. 2005;70(1):51-60.
Mass spectrometric analysis revealed the presence of auxin, mainly in conjugated form, in secretions of Heterodera schachtii and Meloidogyne incognita, with or without treatment with DMT or resorcinol. M. incognita showed the highest production rates, though treatment of M. incognita with resorcinol had a negative effect on auxin production. Analysis of auxin precursor molecules in lysates of H. schachtii, M. incognita and Caenorhabditis elegans suggested that auxin is most probably a degradation product of tryptophan and that auxin may be synthesized via several intermediates, including indole-3-acetamide which is an intermediate of a pathway so far only characterized in bacteria. Furthermore, high levels of anthranilate, a degradation product of tryptophan in animals, but possibly also a precursor for auxin were detected.
质谱分析表明，无论是否用二甲基色胺（DMT）或间苯二酚处理，甜菜孢囊线虫和南方根结线虫的分泌物中均存在生长素，且主要以结合形式存在。南方根结线虫的生长素分泌率最高，不过用间苯二酚处理南方根结线虫会对生长素分泌产生负面影响。对甜菜孢囊线虫、南方根结线虫和秀丽隐杆线虫裂解物中生长素前体分子的分析表明，生长素很可能是色氨酸的降解产物，且生长素可能通过几种中间体合成，包括吲哚 - 3 - 乙酰胺，该中间体是目前仅在细菌中鉴定出的一条途径的中间体。此外，还检测到了动物体内色氨酸的降解产物邻氨基苯甲酸的高水平，但其也可能是生长素的前体。
