Vakgroep Moleculaire Genetica, Departement Plantensysteembiologie, Vlaams Interuniversitair Instituut voor Biotechnologie (VIB), Universiteit Gent, K.L. Ledeganckstraat 35, B-9000 Gent, Belgium;
Mol Plant Pathol. 2003 Jul 1;4(4):271-7. doi: 10.1046/j.1364-3703.2003.00176.x.
SUMMARY The presence of different types of cytokinins was analysed in exudates and lysates of stage-2 juveniles of Heterodera schachtii and Meloidogyne incognita and in mixed stages of Caenorhabditis elegans. For all species, cytokinins were detected in lysates and exudates in which benzyladenine and zeatin-type cytokinins were the most prominent forms. The production of cytokinins by Meloidogyne was much higher than by Heterodera, and the detected levels were in a range which interfered with the physiological activities of the host plant. The presence of 5-methoxy-N,N-dimethyltryptamine hydrogen oxalate did not affect hormone production by H. schachtii, whereas resorcinol slightly stimulated hormone production by M. incognita. The exuded cytokinins may play a role in feeding site induction, more particularly in cell cycle activation and in establishing the feeding site as a nutrient sink.
本研究分析了阶段 2 根结线虫和南方根结线虫幼虫渗出物和裂解物，以及秀丽隐杆线虫混合阶段中不同类型细胞分裂素的存在情况。所有物种的细胞分裂素都在裂解物和渗出物中被检测到，其中苄基腺嘌呤和玉米素型细胞分裂素是最主要的形式。根结线虫产生的细胞分裂素比异皮线虫多得多，而且检测到的水平干扰了宿主植物的生理活动。5-甲氧基-N,N-二甲基色胺草酸盐的存在并不影响异皮线虫细胞分裂素的产生，而间苯二酚则轻微刺激南方根结线虫细胞分裂素的产生。分泌的细胞分裂素可能在取食部位诱导中发挥作用，特别是在细胞周期激活和建立取食部位作为养分汇中发挥作用。
