Takahashi T, Ishihara K, Kojima A, Asai T, Harada K, Tamura Y
National Veterinary Assay Laboratory, Ministry of Agriculture, Forestry and Fisheries, 1-15-1 Tokura, Kokubunji, 185-8511 Tokyo, Japan.
J Vet Med B Infect Dis Vet Public Health. 2005 Dec;52(10):460-4. doi: 10.1111/j.1439-0450.2005.00890.x.
The objective of the present study was to determine whether selection of fluoroquinolone resistance could be easily induced in Campylobacter jejuni-colonized chickens by treatment with enrofloxacin of representative fluoroquinolones at the inherent dosage licensed in Japan (50 ppm in drinking water for 3 days). In the case of isolates from chickens of study 1, an increase in the population of susceptible isolates appeared after the cessation of treatment and maintained throughout the experiments. On the contrary, our results of study 2 demonstrated that administration of enrofloxacin generated a rapid increase of fluoroquinolone resistance in C. jejuni showing the mutation of Asp-90-Asn in the gyrA gene. Present results indicate that the enrofloxacin treatment for broilers at the inherent dosage is able to select fluoroquinolone resistance in C. jejuni. We conclude that whatever enrofloxacin dosage is used, an emergence of fluoroquinolone resistant of C. jejuni occurs.
本研究的目的是确定在日本许可的固有剂量（饮用水中50 ppm，持续3天）下，用恩诺沙星或代表性氟喹诺酮类药物治疗空肠弯曲菌定植的鸡，是否能轻易诱导出氟喹诺酮耐药性。在研究1的鸡分离株中，治疗停止后，敏感分离株的数量增加，并在整个实验过程中保持。相反，我们的研究2结果表明，恩诺沙星给药导致空肠弯曲菌中氟喹诺酮耐药性迅速增加，显示出gyrA基因中Asp-90-Asn的突变。目前的结果表明，以固有剂量对肉鸡进行恩诺沙星治疗能够在空肠弯曲菌中选择出氟喹诺酮耐药性。我们得出结论，无论使用何种恩诺沙星剂量，空肠弯曲菌都会出现氟喹诺酮耐药性。
