Krause Christopher D, Mei Erwen, Mirochnitchenko Olga, Lavnikova Natasha, Xie Junxia, Jia Yiwei, Hochstrasser Robin M, Pestka Sidney
Department of Molecular Genetics, Microbiology, and Immunology, The University of Medicine and Dentistry of New Jersey-Robert Wood Johnson Medical School, 675 Hoes Lane West, Piscataway, NJ 08854, USA.
Biochem Biophys Res Commun. 2006 Feb 10;340(2):377-85. doi: 10.1016/j.bbrc.2005.11.182. Epub 2005 Dec 13.
We used fluorescence resonance energy transfer previously to show that the interferon-gamma (IFN-gamma) receptor complex is a preformed entity mediated by constitutive interactions between the IFN-gammaR2 and IFN-gammaR1 chains, and that this preassembled entity changes its structure after the treatment of cells with IFN-gamma. We applied this technique to determine the structure of the interleukin-10 (IL-10) receptor complex and whether it undergoes a similar conformational change after treatment of cells with IL-10. We report that, like the IFN-gamma receptor complex, the IL-10 receptor complex is preassembled: constitutive but weaker interactions occur between the IL-10R1 and IL-10R2 chains, and between two IL-10R2 chains. The IL-10 receptor complex undergoes no major conformational changes when cells are treated with cellular or Epstein-Barr viral IL-10. Receptor complex preassembly may be an inherent feature of Class 2 cytokine receptor complexes.
我们之前使用荧光共振能量转移来表明，干扰素-γ（IFN-γ）受体复合物是一个预先形成的实体，由IFN-γR2和IFN-γR1链之间的组成性相互作用介导，并且在用IFN-γ处理细胞后，这个预先组装的实体的结构会发生变化。我们应用该技术来确定白细胞介素-10（IL-10）受体复合物的结构，以及在用IL-10处理细胞后它是否会发生类似的构象变化。我们报告，与IFN-γ受体复合物一样，IL-10受体复合物也是预先组装的：IL-10R1和IL-10R2链之间以及两条IL-10R2链之间存在组成性但较弱的相互作用。当用细胞来源的或爱泼斯坦-巴尔病毒的IL-10处理细胞时，IL-10受体复合物不会发生主要的构象变化。受体复合物的预先组装可能是2类细胞因子受体复合物的一个固有特征。
