González-Martínez T, Monjil D F, Aguado-Barrios A, Cobo J, Germanà G, Vega J A
Departamento de Morfología y Biología Celular, Universidad de Oviedo, 33006 Oviedo, Spain.
Neurosci Lett. 2006 Feb 6;394(1):13-6. doi: 10.1016/j.neulet.2005.06.074. Epub 2005 Dec 20.
The occurrence and distribution of Bcl-2, a protein involved in the death-life cell pathways, was investigated in the peripheral sensory nervous system of healthy adult humans, including lumbar dorsal root ganglia, nerve trunks and glabrous skin (to analyze sensory corpuscles) using Western blot and immunohistochemistry. The antibody used labelled a protein of 26 kDa of estimated molecular weight corresponding with Bcl-2. Immunohistochemistry showed that only a neuronal population in dorsal root ganglia, some axons in peripheral nerves and the axon supplying Meissner and Pacinian corpuscles contained Bcl-2, whereas peripheral glial cells (i.e. satellite glial cells, Schwann cell, and lamellar cells of sensory corpuscles) did not. These results suggest that in normal conditions, Bcl-2 is only present in some neuronal, but not glial, elements of the sensory peripheral nervous system. The functional significance, if any, of these results remains to be determined.
利用蛋白质免疫印迹法和免疫组织化学方法，对健康成年人外周感觉神经系统（包括腰段背根神经节、神经干和无毛皮肤（用于分析感觉小体））中参与细胞生死途径的蛋白质Bcl-2的表达和分布情况进行了研究。所用抗体标记了一种估计分子量为26 kDa的蛋白质，与Bcl-2相对应。免疫组织化学显示，只有背根神经节中的一部分神经元、外周神经中的一些轴突以及为迈斯纳小体和环层小体提供神经支配的轴突含有Bcl-2，而外周神经胶质细胞（即卫星胶质细胞、施万细胞和感觉小体的板层细胞）则没有。这些结果表明，在正常情况下，Bcl-2仅存在于感觉外周神经系统的某些神经元成分中，而非神经胶质细胞成分中。这些结果的功能意义（如果有的话）仍有待确定。
