García-Piqueras Jorge, Carcaba Lucia, García-Mesa Yolanda, Feito Jorge, García Beatriz, Viña E, Suárez-Quintanilla Juan, Cobo Juan, Vega Jose A, García-Suárez Olivia
Departamento de Morfología y Biología Celular, Grupo SINPOS Universidad de Oviedo, Oviedo, Spain.
Servicio de Anatomía Patológica, Hospital Universitario de Salamanca, Salamanca, Spain.
Anat Rec (Hoboken). 2019 Feb;302(2):325-331. doi: 10.1002/ar.23951. Epub 2018 Nov 19.
Chondroitin sulfate is a glycosaminoglycan involved in maintaining the morphofunctional properties of the extracellular matrix in peripheral nerves, but its distribution in human sensory corpuscles is unknown despite the role of extracellular matrix in mechanotransduction and axonal guidance. In this study we used immunohistochemistry to analyze the distribution of chondroitin sulfate in human cutaneous Meissner and Pacinian corpuscles. Chondroitin sulfate expression was absent from Meissner corpuscles. In Pacinian corpuscles chondroitin sulfate was found associated to a CD34 positive endoneurial-related layer, interposed between the S100 protein positive inner core cells, and the vimentin positive inner core and outer core-capsule cells. Therefore, the intermediate CD34+/chondroitin sulfate+ intermediate layer present in Pacinian corpuscles isolates the neural segment of the corpuscles (axon and inner core) from the non-neural segments (outer core and capsule). These results suggest a role of chondroitin sulfate in the proper axonal growth and guidance, within the neuronal compartment of the Pacinian corpuscles during development and reinnervation, can be hypothesized. Moreover, a role of CS in mechanotransduction cannot be ruled out. Anat Rec, 302:325-331, 2019. © 2018 Wiley Periodicals, Inc.
硫酸软骨素是一种糖胺聚糖，参与维持外周神经细胞外基质的形态功能特性。尽管细胞外基质在机械转导和轴突导向中发挥作用，但硫酸软骨素在人类感觉小体中的分布尚不清楚。在本研究中，我们使用免疫组织化学方法分析硫酸软骨素在人类皮肤迈斯纳小体和环层小体中的分布。迈斯纳小体中未检测到硫酸软骨素表达。在环层小体中，硫酸软骨素与CD34阳性的神经内膜相关层相关，该层介于S100蛋白阳性的内核细胞与波形蛋白阳性的内核和外核-被膜细胞之间。因此，环层小体中存在的中间CD34+/硫酸软骨素+中间层将小体的神经部分（轴突和内核）与非神经部分（外核和被膜）分隔开来。这些结果表明，硫酸软骨素在发育和再支配过程中，对环层小体神经元部分内轴突的正常生长和导向可能发挥作用。此外，不能排除硫酸软骨素在机械转导中的作用。《解剖学记录》，302:325 - 331，2019年。© 2018威利期刊公司。
