Bulina Maria E, Chudakov Dmitriy M, Britanova Olga V, Yanushevich Yurii G, Staroverov Dmitry B, Chepurnykh Tatyana V, Merzlyak Ekaterina M, Shkrob Maria A, Lukyanov Sergey, Lukyanov Konstantin A
Institute of Bioorganic Chemistry RAS, Miklukho-Maklaya 16/10, Moscow 117997, Russia.
Nat Biotechnol. 2006 Jan;24(1):95-9. doi: 10.1038/nbt1175. Epub 2005 Dec 20.
Photosensitizers are chromophores that generate reactive oxygen species (ROS) upon light irradiation. They are used for inactivation of specific proteins by chromophore-assisted light inactivation (CALI) and for light-induced cell killing in photodynamic therapy. Here we report a genetically encoded photosensitizer, which we call KillerRed, developed from the hydrozoan chromoprotein anm2CP, a homolog of green fluorescent protein (GFP). KillerRed generates ROS upon irradiation with green light. Whereas known photosensitizers must be added to living systems exogenously, KillerRed is fully genetically encoded. We demonstrate the utility of KillerRed for light-induced killing of Escherichia coli and eukaryotic cells and for inactivating fusions to beta-galactosidase and phospholipase Cdelta1 pleckstrin homology domain.
光敏剂是一类发色团，在光照下会产生活性氧（ROS）。它们被用于通过发色团辅助光失活（CALI）使特定蛋白质失活，以及在光动力疗法中用于光诱导细胞杀伤。在此，我们报告一种基因编码的光敏剂，我们将其称为KillerRed，它是由水螅虫纲的发色蛋白anm2CP（绿色荧光蛋白（GFP）的同源物）开发而来。KillerRed在绿光照射下产生活性氧。与已知的光敏剂必须外源添加到生物系统中不同，KillerRed完全由基因编码。我们证明了KillerRed在光诱导杀死大肠杆菌和真核细胞以及使与β-半乳糖苷酶和磷脂酶Cδ1普列克底物蛋白同源结构域的融合蛋白失活方面的效用。
