组成型胞吐作用中膜融合事件的体外重构。胞质蛋白和一种GTP结合蛋白的作用，但钙离子不起作用。
Reconstitution in vitro of a membrane-fusion event involved in constitutive exocytosis. A role for cytosolic proteins and a GTP-binding protein, but not for Ca2+.
作者信息
Edwardson J M, Daniels-Holgate P U
机构信息
Department of Pharmacology, University of Cambridge, U.K.
出版信息
Biochem J. 1992 Jul 15;285 ( Pt 2)(Pt 2):383-5. doi: 10.1042/bj2850383.
Abstract
The fusion of post-Golgi transport vesicles with the plasma membrane is perhaps the least well understood step in the network of intracellular membrane traffic. We have used an 'in vitro' system to study this membrane-fusion event. We show here that fusion requires the presence of cytosolic proteins, but not Ca2+, and is inhibited by the non-hydrolysable GTP analogue guanosine 5'-[gamma-thio]triphosphate, which indicates the involvement of a GTP-binding protein.
摘要
高尔基体后运输小泡与质膜的融合可能是细胞内膜运输网络中最不为人所了解的步骤。我们利用一种“体外”系统来研究这一膜融合事件。我们在此表明，融合需要胞质蛋白的存在，但不需要Ca2+，并且受到不可水解的GTP类似物鸟苷5'-[γ-硫代]三磷酸的抑制，这表明有一个GTP结合蛋白参与其中。
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本文引用的文献
1
A cell-free assay for the insertion of a viral glycoprotein into the plasma membrane.一种用于将病毒糖蛋白插入质膜的无细胞测定法。
J Cell Biol. 1986 Nov;103(5):1829-35. doi: 10.1083/jcb.103.5.1829.
2
A ras-like protein is required for a post-Golgi event in yeast secretion.酵母分泌过程中高尔基体后事件需要一种类Ras蛋白。
Cell. 1987 May 22;49(4):527-38. doi: 10.1016/0092-8674(87)90455-7.
3
Dissection of Semliki Forest virus glycoprotein delivery from the trans-Golgi network to the cell surface in permeabilized BHK cells.在通透的BHK细胞中研究Semliki森林病毒糖蛋白从反式高尔基体网络运输至细胞表面的过程。
Proc Natl Acad Sci U S A. 1988 Nov;85(21):8052-6. doi: 10.1073/pnas.85.21.8052.
4
The yeast GTP-binding YPT1 protein and a mammalian counterpart are associated with the secretion machinery.酵母的 GTP 结合蛋白 YPT1 及其哺乳动物中的对应物与分泌机制相关。
Cell. 1988 Mar 25;52(6):915-24. doi: 10.1016/0092-8674(88)90433-3.
5
Selective recycling of the mannose 6-phosphate/IGF-II receptor to the trans Golgi network in vitro.体外将甘露糖6-磷酸/胰岛素样生长因子-II受体选择性回收至反式高尔基体网络。
Cell. 1988 Oct 21;55(2):309-20. doi: 10.1016/0092-8674(88)90054-2.
6
Do GTPases direct membrane traffic in secretion?GTP 酶是否在分泌过程中指导膜运输？
Cell. 1988 Jun 3;53(5):669-71. doi: 10.1016/0092-8674(88)90081-5.
7
Involvement of GTP-binding "G" proteins in transport through the Golgi stack.GTP结合“G”蛋白参与通过高尔基体堆叠的转运。
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9
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J Cell Biol. 1989 Dec;109(6 Pt 1):2801-8. doi: 10.1083/jcb.109.6.2801.
10
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