de Curtis I, Simons K
European Molecular Biology Laboratory, Heidelberg, Federal Republic of Germany.
Proc Natl Acad Sci U S A. 1988 Nov;85(21):8052-6. doi: 10.1073/pnas.85.21.8052.
In this paper mechanically permeabilized cells have been used to dissect the transport of Semliki Forest virus glycoproteins from the trans-Golgi network to the plasma membrane. Transport from the Golgi complex was monitored by measuring the proteolytic cleavage of the Semliki Forest virus p62 glycoprotein into the E2 and E3 polypeptide chains. Cell surface appearance was measured by the exposure of the exoplasmic domain to antibodies directed against the viral glycoprotein. Both the cleavage of the p62 protein and the transport of the glycoprotein to the cell surface were reconstituted in permeabilized BHK cells when calcium and glucose were present in the medium. Detailed analysis showed that the cleavage of the p62 protein occurred before arrival to the plasma membrane.
在本文中，机械通透细胞已被用于剖析塞姆利基森林病毒糖蛋白从反式高尔基体网络到质膜的转运过程。通过测量塞姆利基森林病毒p62糖蛋白被蛋白水解切割成E2和E3多肽链来监测从高尔基体复合体的转运。通过将胞外结构域暴露于针对病毒糖蛋白的抗体来测量细胞表面的出现情况。当培养基中存在钙和葡萄糖时，p62蛋白的切割以及糖蛋白向细胞表面的转运在通透的BHK细胞中得以重建。详细分析表明，p62蛋白的切割发生在到达质膜之前。
