基于DNA网络模板的银纳米颗粒自组装及其在表面增强拉曼散射中的应用。

DNA-network-templated self-assembly of silver nanoparticles and their application in surface-enhanced Raman scattering.

作者信息

Wei Gang, Wang Li, Liu Zhiguo, Song Yonghai, Sun Lanlan, Yang Tao, Li Zhuang

机构信息

State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Graduate School of the Chinese Academy of Sciences, Chinese Academy of Sciences, Changchun 130022 China.

出版信息

J Phys Chem B. 2005 Dec 22;109(50):23941-7. doi: 10.1021/jp054752x.

DOI:10.1021/jp054752x

PMID:16375382

Abstract

A large-scale lambda-DNA network on a mica surface was successfully fabricated with a simple method. Silver nanoparticles capped with the cationic surfactant cetyltrimethylammonium bromide (CTAB) were self-assembled onto a two-dimensional DNA network template by electrostatic interaction and formed nanoporous silver films, which can be used as active surface-enhanced raman scattering (SERS) substrates. Two probe molecules, Rhodamine 6G (R6G) and 4-aminothiophenol (4-ATP), were studied on these substrates with very low concentrations, and great enhancement factors for R6G (0.21 x 10(10)-4.09 x 10(11)) and 4-ATP (approximately 1.70 x 10(5)) were observed. It was found that the enhancement ability was affected by the DNA concentration and the electrostatic absorption time of the CTAB-stabilized silver nanoparticles on the DNA strands. These SERS substrates formed by the self-assembly of silver nanoparticles on DNA network also show good stability and reproducibility in our experiments.

摘要

通过一种简单的方法成功地在云母表面制备了大规模的λ-DNA网络。用阳离子表面活性剂十六烷基三甲基溴化铵（CTAB）包覆的银纳米颗粒通过静电相互作用自组装到二维DNA网络模板上，形成了纳米多孔银膜，其可用作活性表面增强拉曼散射（SERS）基底。在这些基底上研究了两种探针分子罗丹明6G（R6G）和4-氨基硫酚（4-ATP），它们的浓度非常低，并且观察到R6G（0.21×10¹⁰ - 4.09×10¹¹）和4-ATP（约1.70×10⁵）具有很大的增强因子。发现增强能力受DNA浓度以及CTAB稳定的银纳米颗粒在DNA链上的静电吸附时间的影响。在我们的实验中，由银纳米颗粒在DNA网络上自组装形成的这些SERS基底也显示出良好的稳定性和可重复性。