脂肪酸对骨骼肌代谢和线粒体偶联的急性影响。
Acute effect of fatty acids on metabolism and mitochondrial coupling in skeletal muscle.
作者信息
Hirabara Sandro M, Silveira Leonardo R, Alberici Luciane C, Leandro Carol V G, Lambertucci Rafael H, Polimeno Gisele C, Cury Boaventura Maria F, Procopio Joaquim, Vercesi Anibal E, Curi Rui
机构信息
Department of Physiology and Biophysics, Institute of Biomedical Sciences, University of Sao Paulo, Cidade Universitária, Av. Professor Lineu Prestes, 1524 Butantã, São Paulo, SP 05508-900, Brazil.
出版信息
Biochim Biophys Acta. 2006 Jan;1757(1):57-66. doi: 10.1016/j.bbabio.2005.11.007. Epub 2005 Dec 13.
Acute effects of free fatty acids (FFA) were investigated on: (1) glucose oxidation, and UCP-2 and -3 mRNA and protein levels in 1 h incubated rat soleus and extensor digitorium longus (EDL) muscles, (2) mitochondrial membrane potential in cultured skeletal muscle cells, (3) respiratory activity and transmembrane electrical potential in mitochondria isolated from rat skeletal muscle, and (4) oxygen consumption by anesthetized rats. Long-chain FFA increased both basal and insulin-stimulated glucose oxidation in incubated rat soleus and EDL muscles and reduced mitochondrial membrane potential in C2C12 myotubes and rat skeletal muscle cells. Caprylic, palmitic, oleic, and linoleic acid increased O(2) consumption and decreased electrical membrane potential in isolated mitochondria from rat skeletal muscles. FFA did not alter UCP-2 and -3 mRNA and protein levels in rat soleus and EDL muscles. Palmitic acid increased oxygen consumption by anesthetized rats. These results suggest that long-chain FFA acutely lead to mitochondrial uncoupling in skeletal muscle.
研究了游离脂肪酸（FFA）的急性效应，涉及以下方面：（1）在孵育1小时的大鼠比目鱼肌和趾长伸肌（EDL）中，对葡萄糖氧化、UCP - 2和 - 3 mRNA及蛋白水平的影响；（2）对培养的骨骼肌细胞中线粒体膜电位的影响；（3）对从大鼠骨骼肌分离出的线粒体的呼吸活性和跨膜电位的影响；（4）对麻醉大鼠耗氧量的影响。长链FFA增加了孵育的大鼠比目鱼肌和EDL肌的基础葡萄糖氧化及胰岛素刺激的葡萄糖氧化，并降低了C2C12肌管和大鼠骨骼肌细胞中的线粒体膜电位。辛酸、棕榈酸、油酸和亚油酸增加了大鼠骨骼肌分离线粒体中的耗氧量并降低了膜电位。FFA未改变大鼠比目鱼肌和EDL肌中UCP - 2和 - 3 mRNA及蛋白水平。棕榈酸增加了麻醉大鼠的耗氧量。这些结果表明，长链FFA可急性导致骨骼肌中的线粒体解偶联。
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