Bernard D J, Courjal F, Maurizis J C, Bignon Y J, Chollet P, Plagne R
Unité de Prévention et de Dépistage des Cancers, Centre Jean Perrin, Clermont-Ferrand, France.
Br J Cancer. 1992 Jul;66(1):88-92. doi: 10.1038/bjc.1992.222.
The spontaneous expression of HLA class I and class II molecules in two human breast carcinoma cell lines (MCF7, T47D) and their modulation during epidermal growth factor treatment are reported. Transcription was analysed by Northern blot and hybridisation with HLA class II and class I cDNA specific probes. The expression of cell surface determinants was examined by internal protein labelling with 35s-methionine, immunoprecipitation with monoclonal antibodies specific for HLA class I or class II, followed by isolation of the immune complex on protein A-Sepharose; at least a quantification of glycoprotein was performed by chromatofocusing. Glycoprotein quantification showed a significant increase of HLA class I and class II (DR) antigen expression after stimulation by epidermal growth factor (0.02 microgram ml-1) in the two cell lines, when compared with untreated cell controls. However, with epidermal growth factor treatment of MCF7 and T47D cells, low increases in the amounts of HLA class I and class II RNA were obtained. These differences between expressed antigens and correspondent RNA amounts would be explained by the fact that EGF in these two cell lines acts more in post-transcription for HLA class I and class II antigens.
本文报道了两种人乳腺癌细胞系（MCF7、T47D）中HLA I类和II类分子的自发表达及其在表皮生长因子处理过程中的调节情况。通过Northern印迹法以及与HLA II类和I类cDNA特异性探针杂交来分析转录情况。通过用35s-甲硫氨酸进行内部蛋白质标记、用针对HLA I类或II类的单克隆抗体进行免疫沉淀，随后在蛋白A-琼脂糖上分离免疫复合物来检测细胞表面决定簇的表达；至少通过色谱聚焦法对糖蛋白进行定量。糖蛋白定量显示，与未处理的细胞对照相比，在两种细胞系中，表皮生长因子（0.02微克/毫升）刺激后，HLA I类和II类（DR）抗原表达显著增加。然而，用表皮生长因子处理MCF7和T47D细胞时，HLA I类和II类RNA的量仅有少量增加。表达的抗原与相应RNA量之间的这些差异可以通过以下事实来解释：在这两种细胞系中，表皮生长因子对HLA I类和II类抗原的作用更多地体现在转录后阶段。
