Bernard D J, Maurizis J C, Chassagne J, Chollet P, Plagne R
Anticancer Res. 1986 Jan-Feb;6(1):79-83.
Effects of prolactin on Class II HLA Ag expression have been identified for the first time in a human breast cancer cell line maintained in long-term tissue culture (MCF7) and were reported in this work as follows. Quantification methods for assaying Class II HLA Ag expression modulated by prolactin were established. Class II HLA Ags were internally labelled with [35S] methionine, extracted with Nonidet P-40, immunoprecipitated specifically with anti-Class II HLA MoAbs, isolated on protein A-Sepharose and quantified by chromatofocusing. For low doses of prolactin added to a final concentration (0.015 to 0.350 micrograms/ml culture medium), no change in Class II HLA Ags expressed by MCF7 cells was observed, when compared with controls, the percent of Class II HLA Ags assayed by chromatofocusing was then 4.03 +/- 0.57. For high doses of prolactin added to the final concentration (1.50 micrograms to 3.00 micrograms/ml medium), the percent of Class II HLA Ags increased to 6.05 +/- 0.72. When prolactin was added to the culture medium of MCF7 human breast cancer cell line, increased Class II HLA Ag expression by membrane cells was noted. Prolactin induction of Class II HLA Ag expression by human breast cancer cell lines should prove very useful to study the biology of prolactin in the tumorogenesis of the human breast.
首次在长期组织培养的人乳腺癌细胞系（MCF7）中确定了催乳素对II类HLA抗原表达的影响，本研究报告如下。建立了检测受催乳素调节的II类HLA抗原表达的定量方法。II类HLA抗原用[35S]甲硫氨酸进行内部标记，用Nonidet P - 40提取，用抗II类HLA单克隆抗体进行特异性免疫沉淀，在蛋白A - 琼脂糖上分离并通过层析聚焦进行定量。对于添加到最终浓度（0.015至0.350微克/毫升培养基）的低剂量催乳素，与对照相比，未观察到MCF7细胞表达的II类HLA抗原发生变化，通过层析聚焦测定的II类HLA抗原百分比为4.03±0.57。对于添加到最终浓度（1.50微克至3.00微克/毫升培养基）的高剂量催乳素，II类HLA抗原百分比增加到6.05±0.72。当将催乳素添加到人乳腺癌细胞系MCF7的培养基中时，发现膜细胞的II类HLA抗原表达增加。人乳腺癌细胞系中催乳素诱导II类HLA抗原表达对于研究催乳素在人类乳腺肿瘤发生中的生物学特性应该非常有用。
