Lundbeck F, Overgaard J
Danish Cancer Society, Department of Experimental Clinical Oncology, Aarhus.
Br J Cancer. 1992 Jul;66(1):99-105. doi: 10.1038/bjc.1992.224.
Early and late changes in the reservoir function of the mouse bladder were investigated after radiation alone or a combination of radiation and cisplatinum (cis-DDP). Bladder function was investigated by repeated cystometries. Treatments consisted of either single fraction radiation (5-10-15-25-30 Gy) or 20 Gy in combination with cis-DDP (6 mg kg-1; i.p.) administered at various time intervals from 14 days before until 14 days after radiation. At two selected time intervals (15 min and 4 h before) radiation was given at different dose levels (5-10-15-20 Gy). Within 30 days after irradiation a dose-dependent early response was noticed both in the radiation alone group and the group where cis-DDP was administered 15 min before radiation. The dose-response curve showed a slight but non-significant shift to the left in the combined treatment group (dose effect factor (DEF) = 1.18). Investigation of the early change in bladder reservoir function in the animals treated with 20 Gy alone or a combination of 20 Gy plus cis-DDP at various intervals in relation to irradiation demonstrated a significant increase in response when cis-DDP was administered 24 h and 15 min before and 4 h, 72 h and 336 h after 20 Gy (P less than 0.05). The reversible nature of the early damage was demonstrated. Late response was irreversible and significantly increased in most groups were cis-DDP was administered from 168 h before until 72 h after compared to radiation alone. Comparing groups treated with radiation alone with groups where cis-DDP was administered 15 min and 4 h before radiation revealed DEF values up to 1.45 (P less than 0.05), reflecting the significantly larger response in combined treatment groups. Survival was significantly decreased in all combined treatment groups compared to groups treated with radiation only and likewise survival was decreased in the group treated by cis-DDP alone compared to control (no treatment at all).
研究了单独放疗或放疗与顺铂（顺式二氨基二氯铂）联合治疗后小鼠膀胱储尿功能的早期和晚期变化。通过重复膀胱测压法研究膀胱功能。治疗方案包括单次分割放疗（5 - 10 - 15 - 25 - 30 Gy）或20 Gy联合顺铂（6 mg/kg；腹腔注射），在放疗前14天至放疗后14天的不同时间间隔给药。在两个选定的时间间隔（放疗前15分钟和4小时）给予不同剂量水平（5 - 10 - 15 - 20 Gy）的放疗。照射后30天内，在单独放疗组和放疗前15分钟给予顺铂的组中均观察到剂量依赖性早期反应。剂量反应曲线显示联合治疗组略有但无显著意义的左移（剂量效应因子（DEF） = 1.18）。对单独接受20 Gy照射或20 Gy加顺铂在与照射相关的不同时间间隔联合治疗的动物膀胱储尿功能早期变化的研究表明，当在20 Gy照射前24小时和15分钟以及照射后4小时、72小时和336小时给予顺铂时，反应显著增加（P小于0.05）。证明了早期损伤的可逆性。晚期反应是不可逆的，与单独放疗相比，在大多数在放疗前168小时至放疗后72小时给予顺铂的组中显著增加。将单独放疗组与放疗前15分钟和4小时给予顺铂的组进行比较，DEF值高达1.45（P小于0.05），反映了联合治疗组的反应明显更大。与仅接受放疗的组相比，所有联合治疗组的生存率均显著降低，同样，与对照组（未进行任何治疗）相比，单独接受顺铂治疗的组生存率也降低。
