Green Terry B, Shapiro Alexandra, White Susan, Rao Shujing, Mertens Peter P C, Carner Gerry, Becnel James J
ARS, CMAVE, Gainesville, FL 32608, USA.
J Invertebr Pathol. 2006 Jan;91(1):27-34. doi: 10.1016/j.jip.2005.10.007. Epub 2005 Dec 22.
A cypovirus from the mosquito Culex restuans (named CrCPV) was isolated and its biology, morphology, and molecular characteristics were investigated. CrCPV is characterized by small (0.1-1.0 microm), irregularly shaped inclusion bodies that are multiply embedded. Laboratory studies demonstrated that divalent cations influenced transmission of CrCPV to Culex quinquefasciatus larvae; magnesium enhanced CrCPV transmission by approximately 30% while calcium inhibited transmission. CrCPV is the second cypovirus from a mosquito that has been confirmed by using molecular analysis. CrCPV has a genome composed of 10 dsRNA segments with an electropherotype similar to the recently discovered UsCPV-17 from the mosquito Uranotaenia sapphirina, but distinct from the lepidopteran cypoviruses BmCPV-1 (Bombyx mori) and TnCPV-15 (Trichoplusia ni). Nucleotide and deduced amino acid sequence analysis of CrCPV segment 10 (polyhedrin) suggests that CrCPV is closely related (83% nucleotide sequence identity and 87% amino acid sequence identity) to the newly characterized UsCPV-17 but is unrelated to the 16 remaining CPV species from lepidopteran hosts. A comparison of the terminal segment regions of CrCPV and UsCPV-17, an additional method for differentiating various Cypovirus species, revealed a high level of conservation. Therefore, we propose that CrCPV is a member of the Cypovirus-17 group and designate this species as CrCPV-17.
从致倦库蚊中分离出一种质型多角体病毒（命名为CrCPV），并对其生物学特性、形态学和分子特征进行了研究。CrCPV的特征是具有多个嵌入的小（0.1 - 1.0微米）、形状不规则的包涵体。实验室研究表明，二价阳离子会影响CrCPV向致倦库蚊幼虫的传播；镁可使CrCPV的传播增加约30%，而钙则抑制传播。CrCPV是第二种通过分子分析得到确认的来自蚊子的质型多角体病毒。CrCPV的基因组由10个双链RNA片段组成，其电泳图谱与最近从天蓝优蠓中发现的UsCPV - 17相似，但与鳞翅目质型多角体病毒BmCPV - 1（家蚕）和TnCPV - 15（粉纹夜蛾）不同。对CrCPV第10节段（多角体蛋白）的核苷酸和推导氨基酸序列分析表明，CrCPV与新鉴定的UsCPV - 17密切相关（核苷酸序列同一性为83%，氨基酸序列同一性为87%），但与其余16种来自鳞翅目宿主的CPV物种无关。对CrCPV和UsCPV - 17末端节段区域的比较（这是区分各种质型多角体病毒物种的另一种方法）显示出高度的保守性。因此，我们提议CrCPV是质型多角体病毒 - 17组的成员，并将该物种命名为CrCPV - 17。
