Institute of Microbiology, Jiangxi Academy of Sciences, Nanchang, 330029, Jiangxi, China.
Jiangxi Academy of Sciences, Nanchang, 330029, Jiangxi, China.
Virus Genes. 2023 Dec;59(6):868-873. doi: 10.1007/s11262-023-02029-4. Epub 2023 Sep 12.
Clanis bilineata Walker, soybean hawkmoth, belongs to the subfamily Ambulicinae (Sphingidae, Lepidoptera) and is an edible insect that usually grows on soybean leaves. In this study, we isolated a new cypovirus from naturally diseased Clanis bilineata larvae (named CbCPV), scanned its structure, sequenced its genome, and studied its phylogenetic relationship to other cypoviruses. Microscopy showed that CbCPV polyhedral occlusion bodies were about 1.878 μm on average and contained many virions in the ultrathin sections. The complete genome sequence of CbCPV is 22,812 bp comprising 10 segmented double-stranded RNAs. Apart from segment 1 containing one open reading frame (ORF) and one sub-ORF, the other nine segments all contain one open reading frame and encoded one putative protein. The non-coding regions contained conserved sequences at 5' termini (AGUCAAA) and 3' termini (AGC), except segment 4 containing a different 5' termini (AUGUUUA). The whole sequence of the polyhedrin gene in CbCPV contained 892 nucleotides, encoding a protein of 246 amino acids. Based on amino acid sequences of polyhedrin or RNA dependent RNA polymerase (RdRp), the phylogenetic analysis indicated that CbCPV was closely related to DnCPV-23. The putative function of all segments differed from each other, but the most closely related species of segments were DnCPV-23 with 98.2-99.8% nucleotide identity. Overall, the evidence of morphology, protein analysis and nucleic acids (genomic pattern) showed that CbCPV is a new isolate in the cypovirus-23 type and can be termed Clanis bilineata cypovirus type 23 (CbCPV-23).
双线豆芫菁，大豆天蛾，属于蛱蝶科的 ambulicinae 亚科（鳞翅目），是一种通常生长在大豆叶片上的食用昆虫。在本研究中，我们从自然患病的双线豆芫菁幼虫中分离出一种新的 cypovirus（命名为 CbCPV），扫描了其结构，测序了其基因组，并研究了其与其他 cypovirus 的系统发育关系。显微镜观察显示，CbCPV 多角体约为 1.878 μm，超薄切片中含有许多病毒粒子。CbCPV 的完整基因组序列为 22812 bp，由 10 个分段的双链 RNA 组成。除了包含一个开放阅读框（ORF）和一个亚 ORF 的片段 1 外，其他九个片段都包含一个开放阅读框，并编码一个假定的蛋白质。非编码区在 5' 末端（AGUCAAA）和 3' 末端（AGC）含有保守序列，除了片段 4 含有不同的 5' 末端（AUGUUUA）。CbCPV 多角体基因的全序列包含 892 个核苷酸，编码 246 个氨基酸的蛋白质。基于多角体或 RNA 依赖性 RNA 聚合酶（RdRp）的氨基酸序列，系统发育分析表明，CbCPV 与 DnCPV-23 密切相关。所有片段的假定功能彼此不同，但与片段最密切相关的物种是 DnCPV-23，核苷酸同一性为 98.2-99.8%。总的来说，形态学、蛋白质分析和核酸（基因组模式）的证据表明，CbCPV 是 Cypovirus-23 型的一个新分离株，可以称为双线豆芫菁 Cypovirus 23 型（CbCPV-23）。
