Department of Histology and Embryology, Faculty of Medicine and Dentistry, Palacky University Olomouc, Czech Republic.
Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2022 Sep;166(3):267-273. doi: 10.5507/bp.2021.026. Epub 2021 May 11.
Hofbauer cells (HBCs) are placental macrophages playing various roles during normal and complicated pregnancies, and of the latter, chorioamnionitis is the most frequent.
In placenta with chorioamnionitis, we examined immunohistochemical expression profiles of IL-1β, IL-10, and their potential regulators, CYP2C8 and soluble epoxide hydrolase (sEH), in Hofbauer cells and compared the results with our previously published data for normal placenta.
We found that the expression profiles of the studied proteins in Hofbauer cells in chorioamnionitis differs from normal placenta. In chorioamnionitis, HBCs showed a moderate expression of IL-1β together with a weak expression of IL-10 and CYP2C8. Contrary to normal placenta, HBCs in chorioamnionitis express sEH. We demonstrated a moderate positive correlation between the expression of CYP2C8 and sEH in chorioamnionitis (Spearman r = 0.5654), suggesting enhanced degradation of anti-inflammatory epoxyeicosatrienoic acids. Moreover, the relations of IL-1β and IL-10 to CYP2C8, previously described in normal placenta, disappeared. Furthermore, a weak expression of anti-inflammatory IL-10 in chorioamnionitis was accompanied by change in circularity of HBCs (Spearman r = 0.8193).
Taken together, these findings suggest a possible alteration of the anti-inflammatory role of HBCs and its regulation in chorioamnionitis.
Hofbauer 细胞（HBC）是胎盘巨噬细胞，在正常和复杂妊娠中发挥各种作用，其中绒毛膜羊膜炎最为常见。
在绒毛膜羊膜炎的胎盘组织中，我们检测了 Hofbauer 细胞中白细胞介素-1β（IL-1β）、白细胞介素-10（IL-10）及其潜在调节因子细胞色素 P450 2C8（CYP2C8）和可溶性环氧化物水解酶（sEH）的免疫组织化学表达谱，并将结果与我们之前发表的正常胎盘数据进行了比较。
我们发现，绒毛膜羊膜炎中 Hofbauer 细胞中研究蛋白的表达谱与正常胎盘不同。在绒毛膜羊膜炎中，HBC 表现出中等强度的 IL-1β表达，同时 IL-10 和 CYP2C8 表达较弱。与正常胎盘相反，绒毛膜羊膜炎中的 HBC 表达 sEH。我们证明了绒毛膜羊膜炎中 CYP2C8 和 sEH 的表达之间存在中度正相关（Spearman r = 0.5654），表明抗炎性环氧二十碳三烯酸的降解增强。此外，先前在正常胎盘中描述的 IL-1β和 IL-10 与 CYP2C8 的关系消失。此外，绒毛膜羊膜炎中抗炎性 IL-10 的弱表达伴随着 HBC 形态圆度的变化（Spearman r = 0.8193）。
综上所述，这些发现表明绒毛膜羊膜炎中 Hofbauer 细胞的抗炎作用及其调节可能发生改变。
