Bonney R C, Watson H, Beesley J S, Higham J M, Rogers V, Franks S
Unit of Metabolic Medicine, St Mary's Hospital Medical School, London, UK.
Br J Obstet Gynaecol. 1992 Jun;99(6):486-91. doi: 10.1111/j.1471-0528.1992.tb13787.x.
To compare the activity of calcium independent phospholipase A2 in the endometrium of women with polycystic ovaries and in women with normal ovaries, and to investigate the influence of chronic pelvic pain on phospholipase A2 activity.
A prospective descriptive study.
The Samaritan Hospital for Women and the Unit of Metabolic Medicine, St Mary's Hospital Medical School, London.
73 women attending the Samaritan Hospital for Women for clip sterilization, infertility, early recurrent miscarriage or pelvic venous congestion. 45 of these women had no pelvic pain, 22 had normal ovaries and 23 had polycystic ovaries diagnosed by ultrasound. The other 28 women had chronic pelvic pain, 14 of them had normal ovaries and 14 polycystic ovaries.
Endometrial tissue was obtained at curettage or from the excised uterus in the proliferative or secretory phase of the menstrual cycle. The activities of calcium dependent (type 1) and calcium independent (type 2) phospholipase A2 isoenzymes were measured in all endometrial samples.
In all the women phospholipase A2 type 1 activity, was significantly higher in the secretory phase than in the proliferative phase (P less than 0.001). There was no difference between women with normal ovaries and those with polycystic ovaries at either stage of the cycle irrespective of whether or not chronic pelvic venous congestion was present. In women with normal ovaries, both with and without chronic pelvic pain, phospholipase A2 type 2 activity was significantly higher in the secretory phase than in the proliferative phase (P less than 0.02 and P less than 0.05 respectively) but in women with polycystic ovaries, with and without chronic pelvic pain, there was no significant difference in activity between the two phases of the cycle. Women with polycystic ovaries had markedly higher proliferative phase type 2 activity than women with normal ovaries (P less than 0.001). Secretory phase type 2 activity was similar in all the women investigated.
These data suggest that phospholipase A2 type 2 activity is increased in proliferative phase endometrium of women with polycystic ovaries but that the increase is not associated with chronic pelvic venous congestion.
比较多囊卵巢女性与卵巢正常女性子宫内膜中钙非依赖性磷脂酶A2的活性，并研究慢性盆腔痛对磷脂酶A2活性的影响。
一项前瞻性描述性研究。
撒玛利亚妇女医院以及伦敦圣玛丽医院医学院代谢医学科。
73名因夹子绝育、不孕、早期复发性流产或盆腔静脉淤血而前往撒玛利亚妇女医院就诊的女性。其中45名女性无盆腔痛，22名卵巢正常，23名经超声诊断为多囊卵巢。另外28名女性有慢性盆腔痛，其中14名卵巢正常，14名多囊卵巢。
在月经周期的增殖期或分泌期通过刮宫或切除子宫获取子宫内膜组织。在所有子宫内膜样本中测量钙依赖性（1型）和钙非依赖性（2型）磷脂酶A2同工酶的活性。
在所有女性中，1型磷脂酶A2活性在分泌期显著高于增殖期（P<0.001）。无论是否存在慢性盆腔静脉淤血，在月经周期的任何阶段，卵巢正常的女性与多囊卵巢女性之间均无差异。在卵巢正常的女性中，无论有无慢性盆腔痛，2型磷脂酶A2活性在分泌期均显著高于增殖期（分别为P<0.02和P<0.05），但在多囊卵巢女性中，无论有无慢性盆腔痛，两个月经周期阶段的活性均无显著差异。多囊卵巢女性增殖期2型活性明显高于卵巢正常女性（P<0.001）。在所有研究女性中，分泌期2型活性相似。
这些数据表明，多囊卵巢女性增殖期子宫内膜中2型磷脂酶A2活性增加，但这种增加与慢性盆腔静脉淤血无关。
