Akahoshi Asuka, Koba Kazunori, Ichinose Fumika, Kaneko Mai, Shimoda Asako, Nonaka Kikuko, Yamasaki Masao, Iwata Toshio, Yamauchi Yoshie, Tsutsumi Kentaro, Sugano Michihiro
Faculty of Environmental and Symbiotic Sciences, Prefectural University of Kumamoto, Kumamoto 862-8502, Japan.
Lipids. 2004 Jan;39(1):25-30. doi: 10.1007/s11745-004-1197-3.
The effect of the interaction of CLA and type of dietary protein on lipid metabolism was studied in male rats by feeding diets containing casein (CAS) or soy protein (SOY) as dietary protein and either linoleic acid (LA, a control FA) or graded levels of CLA at 0, 0.1, 0.5, and 1.0% for 28 d. CLA reduced the weight of perirenal adipose tissue in a dose-dependent manner, but the magnitude of the reduction was greater when rats were fed SOY. Feeding SOY resulted in a significant reduction of the concentrations of serum total and HDL cholesterol, TG, glucose, and insulin irrespective of dietary CLA. The concentration of serum leptin tended to be lower on the SOY diet free of CLA than in the corresponding CAS diet, but it fell with an increasing dietary level of CLA in the CAS groups. In contrast, serum leptin tended to increase when CLA was added to SOY diets. The concentration of serum adiponectin was higher in the CAS than in the SOY groups, and it tended to increase in response to dietary CLA levels in the CAS-fed rats, whereas CLA showed no effect in SOY-fed rats. The activity of liver mitochondrial carnitine palmitoyltransferase was higher in the SOY than in the CAS groups, but it tended to increase with an increasing dietary level of CLA in both protein groups. Although the body fat-reducing activity of CLA was more effective when the protein source was SOY, rats fed CAS appeared to be more susceptible to CLA than in those fed SOY with respect to cytokines examined. These results suggest that the type of dietary protein may modify the antiobesity activity of CLA.
通过给雄性大鼠喂食含有酪蛋白（CAS）或大豆蛋白（SOY）作为膳食蛋白质，以及亚油酸（LA，一种对照脂肪酸）或CLA分级水平（0、0.1、0.5和1.0%）的日粮28天，研究了CLA与膳食蛋白质类型的相互作用对脂质代谢的影响。CLA以剂量依赖性方式降低肾周脂肪组织重量，但当给大鼠喂食SOY时，降低幅度更大。无论日粮中CLA如何，喂食SOY都会导致血清总胆固醇、高密度脂蛋白胆固醇、甘油三酯、葡萄糖和胰岛素浓度显著降低。不含CLA的SOY日粮组血清瘦素浓度往往低于相应的CAS日粮组，但在CAS组中，血清瘦素浓度随日粮CLA水平的增加而下降。相反，在SOY日粮中添加CLA时，血清瘦素往往会增加。CAS组血清脂联素浓度高于SOY组，在喂食CAS的大鼠中，血清脂联素浓度往往会随着日粮CLA水平的增加而增加，而CLA对喂食SOY的大鼠没有影响。SOY组肝脏线粒体肉碱棕榈酰转移酶活性高于CAS组，但在两个蛋白质组中，该活性都随着日粮CLA水平的增加而趋于增加。尽管当蛋白质来源为SOY时，CLA的体脂减少活性更有效，但就所检测的细胞因子而言，喂食CAS的大鼠似乎比喂食SOY的大鼠对CLA更敏感。这些结果表明，膳食蛋白质类型可能会改变CLA的抗肥胖活性。
