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龙眼果肉中三种多糖-蛋白复合物的理化特性及免疫调节活性。

Physicochemical characteristics and immunomodulatory activities of three polysaccharide-protein complexes of longan pulp.

机构信息

Ministry of Agriculture/Bio-tech Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510610, China.

出版信息

Molecules. 2011 Jul 21;16(7):6148-64. doi: 10.3390/molecules16076148.

DOI:10.3390/molecules16076148
PMID:21778950
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6264155/
Abstract

Three polysaccharide-protein complexes of longan pulp (LP1-3) were isolated in this work. Their physicochemical characteristics and immunomodulatory effects on splenocytes, natural killer (NK) cells and macrophages in vitro were investigated. The carbohydrate portions of LP1-3 were principally composed of glucose, arabinose and mannose. LP3 displayed the maximal moisture absorption, and the thermal stability of LP2 was obviously higher than that of LP1 and LP3. All of them showed the characteristic polysaccharide and protein bands in the Fourier Transform Infrared (FTIR) spectrum. For a certain dose, all the fractions could significantly stimulate splenocyte proliferation, macrophage phagocytosis against neutral red, and NK cell cytotoxicity against YAC-1 lymphoma cell (P < 0.05). The results demonstrated that the polysaccharide-protein complexes of longan pulp have medical potential as immunotherapeutic adjuvants due to their immunomodulatory activities.

摘要

本文从龙眼果肉中分离得到三种多糖-蛋白复合物（LP1-3）。研究了它们的理化特性以及对体外脾细胞、自然杀伤（NK）细胞和巨噬细胞的免疫调节作用。LP1-3 的糖部分主要由葡萄糖、阿拉伯糖和甘露糖组成。LP3 具有最大的吸湿能力，LP2 的热稳定性明显高于 LP1 和 LP3。它们在傅里叶变换红外（FTIR）光谱中均显示出特征多糖和蛋白带。对于一定剂量，所有这些分数都能显著刺激脾细胞增殖、巨噬细胞吞噬中性红以及 NK 细胞对 YAC-1 淋巴瘤细胞的细胞毒性（P<0.05）。结果表明，由于具有免疫调节活性，龙眼果肉中的多糖-蛋白复合物具有作为免疫治疗佐剂的医学潜力。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/86b9/6264155/6d2053bc520f/molecules-16-06148-g007.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/86b9/6264155/6d2053bc520f/molecules-16-06148-g007.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/86b9/6264155/c5e5e9016502/molecules-16-06148-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/86b9/6264155/69cef594b07f/molecules-16-06148-g003.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/86b9/6264155/5d57169c025b/molecules-16-06148-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/86b9/6264155/6d2053bc520f/molecules-16-06148-g007.jpg

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