Han Chun-Mei, Zhang Jia-Bao, Gao Qing-Hua, Chen Qing-Bo
College of Animal Science and Technology, Talim University, Alar, Xingjiang Autonomous Region, Xinjiang 843300, China.
Yi Chuan. 2005 Nov;27(6):903-7.
Thirteen microsatellite markers, including Sat2,Sat3,Sat4,Sat5,Sat7,Sat8,Sat12, Sat13, Sat16,Sol08,Sol28,Sol30 and Sol03, were studied for their parentage-testing application in a group of 30 JIRONG Rabbits in the present report. The 13 microsatellite loci were successfully amplified with specific primers designed according to known sequences. The PCR products amplified from the microsatellite loci were analyzed by 8% denaturing polyacrylamide gel electrophoresis. The results demonstrated that the average alleles and the mean heterozygosity (Hs) and the polymorphism information content (PIC) and the combined exclusion probability (PE2) of the 13 microsatellite loci were 3.46, 0.578, 0.531, and 0.999329, respectively. The exclusion probability (PE1) of the 13 loci was 0.935226, and the confidence of the parentage testing was less than 80% when data of both parents were unknown, while the exclusion probability (PE1) and the confidence were 0.999329 and 95% respectively with known data of a single parent. Since the data of the rabbit maternal lines studied were known, the paternal lines of the group were successfully identified using the 13 microsatellite loci with high confidence.
在本报告中，研究了包括Sat2、Sat3、Sat4、Sat5、Sat7、Sat8、Sat12、Sat13、Sat16、Sol08、Sol28、Sol30和Sol03在内的13个微卫星标记在30只吉戎兔群体中的亲子鉴定应用。根据已知序列设计特异性引物，成功扩增出13个微卫星位点。对微卫星位点扩增的PCR产物进行8%变性聚丙烯酰胺凝胶电泳分析。结果表明，13个微卫星位点的平均等位基因数、平均杂合度（Hs）、多态信息含量（PIC）和累积排除概率（PE2）分别为3.46、0.578、0.531和0.999329。13个位点的排除概率（PE1）为0.935226，当双亲数据均未知时，亲子鉴定的置信度低于80%，而当单亲数据已知时，排除概率（PE1）和置信度分别为0.999329和95%。由于所研究的兔母系数据已知，利用这13个微卫星位点成功地高置信度鉴定了该群体的父系。
