Chen Min-Li, Zhu Liang, Cai Yue-Qin, Yu Jia, Xu Xiao-Ping, Tu Jue, Xiao Hui, Pan Yong-Ming
Zhejiang Chinese Medical University, Hangzhou 310053, China.
Dongwuxue Yanjiu. 2010 Aug;31(4):401-7. doi: 10.3724/SP.J.1141.2010.04401.
Microsatellite polymorphisms were analyzed in F5, F6 and F7 WHBE rabbits (Oryctolagus cuniculus) to monitor inbreeding. Out of 21 microsatellite loci, 11 were successfully amplified and showed polymorphic. For the F5 WHBE rabbits, the number of alleles per locus (Na) ranged from 3 to 9 and the mean effective number of alleles (Ne) was 1.81. The mean value of observed heterozygosity (Ho) and polymorphism information content (PIC) of the 11 polymorphic loci were 0.381 and 0.524, respectively. The power of cumulative discrimination (CDP) was 1.0. The value of cumulative exclusion probability of the 11 polymorphic loci in the absence (CPE-1) or in the presence of genetic information on the first parent (CPE-2) was 0.926 and 0.993, respectively. For the F6 WHBE rabbits, Na per locus ranged from 3 to 8 and the mean value of Ne was 1.68. The mean value of Ho, PIC, CDP, CPE-1, CPE-2 of the 11 polymorphic loci were 0.356, 0.548, 1.0, 0.931, 0.994 respectively. For the F7 WHBE rabbits, Na per locus ranged from 2 to 6 and the mean value of Ne was 1.51. The mean value of Ho, PIC, CDP, CPE-1, CPE-2 of the 11 polymorphic loci were 0.287, 0.498, 1.0, 0.891, 0.986 respectively. The average number of effective alleles and the average observed heterozygosity were decreasing continuously in F5, F6 and F7, suggesting that the purity of WHBE rabbits was increasing continuously.
对F5、F6和F7代皖南白背兔（穴兔）的微卫星多态性进行分析以监测近亲繁殖情况。在21个微卫星位点中，11个成功扩增并显示出多态性。对于F5代皖南白背兔，每个位点的等位基因数（Na）范围为3至9，平均有效等位基因数（Ne）为1.81。11个多态性位点的观察杂合度（Ho）和多态性信息含量（PIC）的平均值分别为0.381和0.524。累积鉴别力（CDP）为1.0。11个多态性位点在无（CPE - 1）或有第一亲本遗传信息（CPE - 2）时的累积排除概率值分别为0.926和0.993。对于F6代皖南白背兔，每个位点的Na范围为3至8，Ne的平均值为1.68。11个多态性位点的Ho、PIC、CDP、CPE - 1、CPE - 2的平均值分别为0.356、0.548、1.0、0.931、0.994。对于F7代皖南白背兔，每个位点的Na范围为2至6，Ne的平均值为1.51。11个多态性位点的Ho、PIC、CDP、CPE - 1、CPE - 2的平均值分别为0.287、0.498、1.0、0.891、0.986。F5、F6和F7代的平均有效等位基因数和平均观察杂合度持续下降，表明皖南白背兔的纯度在不断提高。
