Rainey G Jonah A, Coffin John M
Department of Biochemistry, Tufts University School of Medicine, Boston, MA, USA.
J Virol. 2006 Jan;80(2):562-70. doi: 10.1128/JVI.80.2.562-570.2006.
The ability of many retroviruses to cause disease can be correlated to their cytopathic effect (CPE) in tissue culture characterized by an acute period of cell death and viral DNA accumulation. Here, we show that mutants of a subgroup B avian retrovirus (Alpharetrovirus) cause a very dramatic CPE in certain susceptible avian cells that is coincident with elevated levels of apoptosis, as measured by nuclear morphology, and persistent viral DNA accumulation. These mutants also have a broadly extended host range that includes rodent, cat, dog, monkey, and human cells (31). Previously, we have shown that the mutants exhibit diminished resistance to superinfection. The results presented here have important implications for the process of evolution of retroviruses to use distinct cellular receptors.
许多逆转录病毒引发疾病的能力与其在组织培养中的细胞病变效应（CPE）相关，其特征为细胞死亡急性期和病毒DNA积累。在此，我们表明B亚群禽逆转录病毒（α逆转录病毒）的突变体在某些易感禽细胞中会引发非常显著的细胞病变效应，这与通过核形态学测量的凋亡水平升高以及持续的病毒DNA积累相一致。这些突变体还具有广泛扩展的宿主范围，包括啮齿动物、猫、狗、猴和人类细胞（31）。此前，我们已表明这些突变体对超感染的抗性降低。此处呈现的结果对于逆转录病毒利用不同细胞受体的进化过程具有重要意义。
