Zhang Fang, Zhang Qian-qian, Zhu Chen-jian, Wang Xiu-lin
College of Chemistry and Chemical Engineering, Ocean University of China, Qingdao 266003, China.
Guang Pu Xue Yu Guang Pu Fen Xi. 2005 Sep;25(9):1439-42.
A new complex [Cu (Thr)(Phen)H2O] SO4 . HO2 x CH3 OH (Thr = DL-Threonine, Phen = o-Phenanthroline), which has not been published, was synthesized and characterized by elemental analysis, IR spectroscopy, and TG-DTA. The interaction of the complex and sperm DNA was studied by electronic absorption and ethidium bromide (EB) fluorescence spectroscopy. The result indicates that the maximal absorption peaks of this complex are red-shifted and the intensity is weakened; At the same time, it can to some extent quench the emission intensity of EB-DNA system. Therefore, the authors come to a conclusion that the interaction of this complex and sperm DNA is intercalation.
合成了一种尚未见报道的新型配合物[Cu(Thr)(Phen)H₂O]SO₄·H₂O·CH₃OH（Thr = DL-苏氨酸，Phen = 邻菲罗啉），并通过元素分析、红外光谱和热重-差热分析对其进行了表征。采用电子吸收光谱和溴化乙锭（EB）荧光光谱研究了该配合物与精子DNA的相互作用。结果表明，该配合物的最大吸收峰发生红移且强度减弱；同时，它能在一定程度上猝灭EB-DNA体系的发射强度。因此，作者得出结论，该配合物与精子DNA的相互作用为嵌入作用。
