Zhang Fang, Zhang Qian-qian, Lu Xiao-lan, Jiang Tao, Wang Xiu-lin
College of Chemistry and Chemical Engineering, Ocean University of China, Qingdao 266003, China.
Guang Pu Xue Yu Guang Pu Fen Xi. 2007 Feb;27(2):302-5.
Two novel complexes of [Cu(HAsp)ImH2O]SO4 x 4 H2O and [Cu(Asp)Im(OH)] x 4 H2O (HAsp = Aspartic acid molecule, Asp = Aspartic acid ion, Im = Imidazole) were synthesized and characterized by elemental analysis, IR spectroscopy and TG-DTA. The interactions of the complexes with sperm DNA were studied by electronic absorption and fluorescence spectra. Results showed that the reactions of the two complexes with DNA are obviously different: for [Cu(HAsp)ImH2O]SO4 x 4 H2O, it is intercalation companied by electrostatic effect, while [Cu(Asp)Im(OH)] x 4 H2O mainly cooperates with the nitrogen atom of the DNA base pair, which induces the breakage of DNA double helix. The reasons for these differences in their DNA binding modes were also discussed.
合成了两种新型配合物[Cu(HAsp)ImH₂O]SO₄·4H₂O和[Cu(Asp)Im(OH)]·4H₂O（HAsp = 天冬氨酸分子，Asp = 天冬氨酸离子，Im = 咪唑），并通过元素分析、红外光谱和热重-差热分析对其进行了表征。通过电子吸收光谱和荧光光谱研究了配合物与精子DNA的相互作用。结果表明，两种配合物与DNA的反应明显不同：对于[Cu(HAsp)ImH₂O]SO₄·4H₂O，其为嵌入作用并伴有静电效应，而[Cu(Asp)Im(OH)]·4H₂O主要与DNA碱基对的氮原子配位，从而导致DNA双螺旋结构的断裂。还讨论了它们在DNA结合模式上存在这些差异的原因。
