鼠伤寒沙门氏菌中pyr基因表达的调控：尿苷和胞苷核苷酸库变化的影响。
Control of expression of the pyr genes in Salmonella typhimurium: effects of variations in uridine and cytidine nucleotide pools.
作者信息
Schwartz M, Neuhard J
出版信息
J Bacteriol. 1975 Mar;121(3):814-22. doi: 10.1128/jb.121.3.814-822.1975.
Abstract
The differential rate of synthesis of five of the pyrimidine biosynthetic enzymes coded for by pyrB-F, and the endogenous concentrations of the individual pyrimidine nucleotides were determined in specially constructed mutants of Salmonella typhimurium. In the mutants employed the different pyrimidine nucleotide pools may be manipulated individually during exponential growth. The results obtained indicate the following. (i) The expression of pyrB, pyrE, and pyrF is controlled by a uridine nucleotide in a noncoordinate manner. (ii) The expression of pyrC and pyrD is regulated predominantly by a cytidine nucleotide. Under all conditions investigated, their expression seems to be coordinated, even though the genes are not contiguous on the chromosome. (iii) The low-molecular-weight effectors involved in controlling the expression of the pyr genes are neither uridine 5'-monophosphate nor cytidine 5'-monophosphate, but rather the corresponding di- or triphosphates.
摘要
在专门构建的鼠伤寒沙门氏菌突变体中，测定了由pyrB - F编码的五种嘧啶生物合成酶的合成差异速率以及各个嘧啶核苷酸的内源性浓度。在所使用的突变体中，不同的嘧啶核苷酸库在指数生长期间可被单独操纵。所得结果表明如下：（i）pyrB、pyrE和pyrF的表达由尿苷核苷酸以非协同方式控制。（ii）pyrC和pyrD的表达主要由胞苷核苷酸调节。在所有研究条件下，它们的表达似乎是协同的，尽管这些基因在染色体上并不相邻。（iii）参与控制pyr基因表达的低分子量效应物既不是尿苷5'-单磷酸也不是胞苷5'-单磷酸，而是相应的二磷酸或三磷酸。
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Control of expression of the pyr genes in Salmonella typhimurium: effects of variations in uridine and cytidine nucleotide pools.鼠伤寒沙门氏菌中pyr基因表达的调控：尿苷和胞苷核苷酸库变化的影响。
J Bacteriol. 1975 Mar;121(3):814-22. doi: 10.1128/jb.121.3.814-822.1975.
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