Nakano Mayumi, Nobuta Kan, Vemaraju Kalyan, Tej Shivakundan Singh, Skogen Jeremy W, Meyers Blake C
Department of Plant and Soil Sciences, University of Delaware, Newark, DE 19714, USA.
Nucleic Acids Res. 2006 Jan 1;34(Database issue):D731-5. doi: 10.1093/nar/gkj077.
MPSS (massively parallel signature sequencing) is a sequencing-based technology that uses a unique method to quantify gene expression level, generating millions of short sequence tags per library. We have created a series of databases for four species (Arabidopsis, rice, grape and Magnaporthe grisea, the rice blast fungus). Our MPSS databases measure the expression level of most genes under defined conditions and provide information about potentially novel transcripts (antisense transcripts, alternative splice isoforms and regulatory intergenic transcripts). A modified version of MPSS has been used to perform deep profiling of small RNAs from Arabidopsis, and we have recently adapted our database to display these data. Interpretation of the small RNA MPSS data is facilitated by the inclusion of extensive repeat data in our genome viewer. All the data and the tools introduced in this article are available at http://mpss.udel.edu.
大规模平行签名测序（MPSS）是一种基于测序的技术，它采用独特方法来定量基因表达水平，每个文库可生成数百万个短序列标签。我们已针对四个物种（拟南芥、水稻、葡萄和稻瘟病菌Magnaporthe grisea）创建了一系列数据库。我们的MPSS数据库可测量特定条件下大多数基因的表达水平，并提供有关潜在新转录本（反义转录本、可变剪接异构体和调控基因间转录本）的信息。MPSS的一个改进版本已用于对拟南芥中的小RNA进行深度分析，并且我们最近对数据库进行了调整以展示这些数据。通过在我们的基因组浏览器中纳入大量重复数据，有助于对小RNA MPSS数据进行解读。本文介绍的所有数据和工具均可在http://mpss.udel.edu获取。
