Yamashita Riu, Suzuki Yutaka, Wakaguri Hiroyuki, Tsuritani Katsuki, Nakai Kenta, Sugano Sumio
Human Genome Center, Institute of Medical Science, University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108-8639, Japan.
Nucleic Acids Res. 2006 Jan 1;34(Database issue):D86-9. doi: 10.1093/nar/gkj129.
DBTSS was first constructed in 2002 based on precise, experimentally determined 5' end clones. Several major updates and additions have been made since the last report. First, the number of human clones has drastically increased, going from 190,964 to 1,359,000. Second, information about potential alternative promoters is presented because the number of 5' end clones is now sufficient to determine several promoters for one gene. Namely, we defined putative promoter groups by clustering transcription start sites (TSSs) separated by <500 bases. A total of 8308 human genes and 4276 mouse genes were found to have putative multiple promoters. Third, DBTSS provides detailed sequence comparisons of user-specified TSSs. Finally, we have added TSS information for zebrafish, malaria and schyzon (a red algae model organism). DBTSS is accessible at http://dbtss.hgc.jp.
DBTSS最初于2002年基于精确的、经实验确定的5'端克隆构建而成。自上次报告以来，已进行了几次重大更新和补充。首先，人类克隆的数量大幅增加，从190,964个增至1,359,000个。其次，由于现在5'端克隆的数量足以确定一个基因的多个启动子，因此提供了有关潜在替代启动子的信息。具体而言，我们通过对间隔小于500个碱基的转录起始位点（TSS）进行聚类来定义推定的启动子组。总共发现8308个人类基因和4276个小鼠基因具有推定的多个启动子。第三，DBTSS提供用户指定的TSS的详细序列比较。最后，我们添加了斑马鱼、疟疾和裂殖壶菌（一种红藻模式生物）的TSS信息。可通过http://dbtss.hgc.jp访问DBTSS。
