Hale A H, Winkelhake J L, Weber M J
J Cell Biol. 1975 Feb;64(2):398-407. doi: 10.1083/jcb.64.2.398.
We have investigated whether cell surface changes associated with growth control and malignant transformation are linked to the cell cycle. Chicken embryo cells synchronized by double thymidine block were examined for cell-cycle-dependent alterations in membrane function (measured by transport of 2-deoxyglucose, uridine, thymidine, and mannitol), in cell surface morphology (examined by scanning electron microscopy), and in the ability of tumor virus gene expression to induce a transformation-specific change in membrane function. We reach the following conclusions: (a) The high rate of 2-deoxyglucose transport seen in transformed cells and the low rates of 2-deoxyglucose and uridine transport characteristic of density-inhibited cells do not occur in normal growing cells as they traverse the cell cycle. (b) Although there are cell cycle-dependent changes in surface morphology, they are not reflected in corresponding changes in membrane function. (c) Tumor virus gene expression can alter cell membrane function at any stage in the cell cycle and without progression through the cell cycle.
我们研究了与生长控制和恶性转化相关的细胞表面变化是否与细胞周期有关。对通过双胸腺嘧啶核苷阻断同步化的鸡胚细胞进行了检查，以观察其在膜功能（通过2-脱氧葡萄糖、尿苷、胸腺嘧啶核苷和甘露醇的转运来测量）、细胞表面形态（通过扫描电子显微镜检查）以及肿瘤病毒基因表达诱导膜功能发生转化特异性变化的能力方面的细胞周期依赖性改变。我们得出以下结论：（a）正常生长的细胞在经历细胞周期时，不会出现转化细胞中所见的高2-脱氧葡萄糖转运速率以及密度抑制细胞特有的低2-脱氧葡萄糖和尿苷转运速率。（b）尽管细胞表面形态存在细胞周期依赖性变化，但它们并未反映在膜功能的相应变化中。（c）肿瘤病毒基因表达可在细胞周期的任何阶段改变细胞膜功能，且无需经历细胞周期进程。
