密度抑制的鸡胚细胞培养物中葡萄糖转运的刺激作用。
Stimulation of glucose transport in cultures of density-inhibited chick embryo cells.
作者信息
Sefton B M, Rubin H
出版信息
Proc Natl Acad Sci U S A. 1971 Dec;68(12):3154-7. doi: 10.1073/pnas.68.12.3154.
Abstract
The rate of glucose transport in sparse, rapidly growing chick-embryo fibroblasts is much greater than that in density-inhibited cells. The addition of fresh chicken serum or trypsin to the medium of density-inhibited cells causes a large increase in the rate of glucose transport that is detectable 15 min after addition. The increase in glucose transport precedes the increase in DNA synthesis by 5-6 hr. Only small changes in rates of transport are seen with nucleosides or a nonmetabolizable amino acid. The increase in glucose transport requires protein synthesis but not RNA or DNA synthesis.
摘要
在稀疏、快速生长的鸡胚成纤维细胞中，葡萄糖转运速率远高于密度抑制细胞。向密度抑制细胞的培养基中添加新鲜鸡血清或胰蛋白酶会导致葡萄糖转运速率大幅增加，添加后15分钟即可检测到。葡萄糖转运的增加比DNA合成的增加早5至6小时。核苷或不可代谢氨基酸只会使转运速率发生微小变化。葡萄糖转运的增加需要蛋白质合成，但不需要RNA或DNA合成。
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