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Stimulation of glucose transport in cultures of density-inhibited chick embryo cells.密度抑制的鸡胚细胞培养物中葡萄糖转运的刺激作用。
Proc Natl Acad Sci U S A. 1971 Dec;68(12):3154-7. doi: 10.1073/pnas.68.12.3154.
2
Hexose and amino acid transport by chicken embryo fibroblasts infected with temperature-sensitive mutant of Rous sarcoma virus. Comparison of transport properties of whole cells and membrane vesicles.感染劳斯肉瘤病毒温度敏感突变体的鸡胚成纤维细胞对己糖和氨基酸的转运。全细胞与膜泡转运特性的比较。
Biochim Biophys Acta. 1980 Jun 6;598(3):616-27. doi: 10.1016/0005-2736(80)90041-3.
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Early cellular responses to diverse growth stimuli independent of protein and RNA synthesis.早期细胞对多种生长刺激的反应，与蛋白质和RNA合成无关。
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Loss of the post-translational control of nutrient transport in vitro and in vivo virus-transformed chicken cells.体外和体内病毒转化的鸡细胞中营养物质运输的翻译后控制丧失。
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Stimulation of density-inhibited cell cultures by insulin.胰岛素对密度抑制细胞培养物的刺激作用。
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Inhibition of DNA synthesis in chick embryo cultures by deprivation of either serum or zinc.通过剥夺血清或锌来抑制鸡胚培养物中的DNA合成。
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Tyrosine phosphorylation of specific proteins after mitogen stimulation of chicken embryo fibroblasts.丝裂原刺激鸡胚成纤维细胞后特定蛋白质的酪氨酸磷酸化
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Effects of combined glutamine and serum deprivation on glucose control of hexose transport in mammalian fibroblast cultures.谷氨酰胺与血清剥夺联合作用对哺乳动物成纤维细胞培养物中己糖转运葡萄糖控制的影响。
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Inhibition of DNA synthesis in chick embryo cultures by deprivation of either serum or zinc.通过剥夺血清或锌来抑制鸡胚培养物中的DNA合成。
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Growth-and density-dependent inhibition of deoxyglucose transport in Balb 3T3 cells and its absence in cells transformed by murine sarcoma virus.Balb 3T3细胞中脱氧葡萄糖转运的生长和密度依赖性抑制及其在鼠肉瘤病毒转化细胞中的缺失。
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本文引用的文献

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Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
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2
Studies of tissue permeability. V. The penetration and phosphorylation of 2-deoxyglucose in the rat diaphragm.组织通透性研究。V. 2-脱氧葡萄糖在大鼠膈肌中的渗透与磷酸化
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Extrogen control of uterine glucose metabolism. An analysis based on the transport and phosphorylation of 2-deoxyglucose.子宫葡萄糖代谢的雌激素调控。基于2-脱氧葡萄糖转运和磷酸化的分析。
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Effects of local cell concentrations upon the growth of chick embryo cells in tissue culture.局部细胞浓度对组织培养中鸡胚细胞生长的影响。
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Release from density dependent growth inhibition by proteolytic enzymes.通过蛋白水解酶从密度依赖性生长抑制中释放出来。
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Local stimulation of growth in primary cultures of chick embryo fibroblasts.鸡胚成纤维细胞原代培养中生长的局部刺激
Proc Natl Acad Sci U S A. 1969 Mar;62(3):906-11. doi: 10.1073/pnas.62.3.906.
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Uridine transport and RNA synthesis in growing and in density-inhibited animal cells.生长中和密度抑制的动物细胞中的尿苷转运与RNA合成
J Cell Physiol. 1971 Apr;77(2):157-68. doi: 10.1002/jcp.1040770205.
8
Analysis of a functional change in membrane in the process of cell transformation by Rous sarcoma virus; alteration in the characteristics of sugar transport.劳氏肉瘤病毒诱导细胞转化过程中细胞膜功能变化的分析；糖转运特性的改变。
Virology. 1970 Aug;41(4):647-52. doi: 10.1016/0042-6822(70)90429-0.
9
Evidence for a functional change in the plasma membrane of murine sarcoma virus-infected mouse embryo cells. Transport and transport-associated phosphorylation of 14C-2-deoxy-D-glucose.鼠肉瘤病毒感染的小鼠胚胎细胞质膜功能变化的证据。14C-2-脱氧-D-葡萄糖的转运及与转运相关的磷酸化作用。
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10
Transport changes rapidly initiated by serum addition to "contact inhibited" 3T3 cells.向“接触抑制”的3T3细胞中添加血清后迅速引发转运变化。
Proc Natl Acad Sci U S A. 1969 Nov;64(3):1049-56. doi: 10.1073/pnas.64.3.1049.

密度抑制的鸡胚细胞培养物中葡萄糖转运的刺激作用。

Stimulation of glucose transport in cultures of density-inhibited chick embryo cells.

作者信息

Sefton B M, Rubin H

出版信息

Proc Natl Acad Sci U S A. 1971 Dec;68(12):3154-7. doi: 10.1073/pnas.68.12.3154.

DOI:10.1073/pnas.68.12.3154
PMID:5289253
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC389611/
Abstract

The rate of glucose transport in sparse, rapidly growing chick-embryo fibroblasts is much greater than that in density-inhibited cells. The addition of fresh chicken serum or trypsin to the medium of density-inhibited cells causes a large increase in the rate of glucose transport that is detectable 15 min after addition. The increase in glucose transport precedes the increase in DNA synthesis by 5-6 hr. Only small changes in rates of transport are seen with nucleosides or a nonmetabolizable amino acid. The increase in glucose transport requires protein synthesis but not RNA or DNA synthesis.

摘要

在稀疏、快速生长的鸡胚成纤维细胞中，葡萄糖转运速率远高于密度抑制细胞。向密度抑制细胞的培养基中添加新鲜鸡血清或胰蛋白酶会导致葡萄糖转运速率大幅增加，添加后15分钟即可检测到。葡萄糖转运的增加比DNA合成的增加早5至6小时。核苷或不可代谢氨基酸只会使转运速率发生微小变化。葡萄糖转运的增加需要蛋白质合成，但不需要RNA或DNA合成。