Ambros V R, Chen L B, Buchanan J M
Proc Natl Acad Sci U S A. 1975 Aug;72(8):3144-8. doi: 10.1073/pnas.72.8.3144.
Confluent chick embryo fibroblasts infected with the Ts68 mutant of Rous sarcoma virus were examined by scanning electron microscopy at the permissive (36 degrees) and nonpermissive (41 degrees) temperatures for transformation. Infected cells shifted from 41 degrees to 36 degrees undergo a change in shape from elongated to rounded. This process is preceded by the appearance of surface ruffles on the cell. These surface ruffles are not observed on cells maintained at 41 degrees, appear as early as 0.5 hr after a shift to 36 degrees, and are common on cells maintained at 36 degrees. By 3.5 hr after the shift from 41 degrees to 36 degrees, cultures appear fully transformed by the criteria of cell roundedness and the presence of surface ruffles. This surface alteration of cells is the earliest event of those so far reported during the transformation process and is not dependent upon protein synthesis and extracellular plasminogen during the period of temperature shift.
用扫描电子显微镜在允许转化的温度（36摄氏度）和不允许转化的温度（41摄氏度）下，对感染劳斯肉瘤病毒Ts68突变体的融合鸡胚成纤维细胞进行了检查。从41摄氏度转移到36摄氏度的感染细胞会经历从细长形到圆形的形态变化。在此过程之前，细胞表面会出现褶皱。在维持于41摄氏度的细胞上未观察到这些表面褶皱，转移到36摄氏度后最早在0.5小时出现，并且在维持于36摄氏度的细胞上很常见。从41摄氏度转移到36摄氏度后3.5小时，根据细胞圆形度和表面褶皱的存在标准，培养物似乎完全转化。细胞的这种表面改变是迄今为止在转化过程中报道的最早事件，并且在温度转移期间不依赖于蛋白质合成和细胞外纤溶酶原。
