Leung Lai-Yee, Tong Kai-Yu, Zhang Shao-Min, Zeng Xiang-Hui, Zhang Ke-Pin, Zheng Xiao-Xiang
Department of Health Technology and Informatics, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong.
Neurosci Lett. 2006;397(1-2):135-9. doi: 10.1016/j.neulet.2005.12.006. Epub 2005 Dec 27.
Treadmill exercise and neuromuscular electrical stimulation are common clinical approaches for stroke rehabilitation. Both animal and clinical studies have shown the functional improvements after these interventions. However, the neurochemical effects on the ischemic brain had not been well studied. This study aimed at evaluating the effects of treadmill exercise and neuromuscular electrical stimulation (NMES), and studying their effects during a 2-week training, on the levels of common neurotransmitters (aspartate, glutamate, taurine and gamma-aminobutyric acid (GABA)) in the hippocampus following transient focal cerebral ischemia. Either treadmill exercise or neuromuscular electrical stimulation was prescribed to the rats 24 h after cerebral ischemia whereas Control group remained in cages for 2 weeks. Microdialysis technique was used to collect dialysates from ipsilesional hippocampus in vivo. It was found that the glutamate level was increased significantly during treadmill exercise and then returned to baseline level. Both interventions did not trigger significant effects on aspartate and glutamate basal levels during the 2 weeks. The relatively high taurine level in Control groups may suggest that the interventions might suppress the taurine release in hippocampus. GABA and aspartate levels did not showed significant changes over the 2 weeks in all groups. These results provide insights to explain the neurochemical effects on the ischemic injured brain during the course of rehabilitation.
跑步机运动和神经肌肉电刺激是中风康复的常见临床方法。动物和临床研究均表明，这些干预措施后功能有所改善。然而，对缺血性脑的神经化学影响尚未得到充分研究。本研究旨在评估跑步机运动和神经肌肉电刺激（NMES）的效果，并研究在为期2周的训练期间，它们对短暂性局灶性脑缺血后海马体中常见神经递质（天冬氨酸、谷氨酸、牛磺酸和γ-氨基丁酸（GABA））水平的影响。在脑缺血24小时后，对大鼠进行跑步机运动或神经肌肉电刺激，而对照组则在笼中饲养2周。采用微透析技术在体内收集患侧海马体的透析液。结果发现，跑步机运动期间谷氨酸水平显著升高，然后恢复到基线水平。在这2周内，两种干预措施对天冬氨酸和谷氨酸的基础水平均未产生显著影响。对照组中相对较高的牛磺酸水平可能表明，这些干预措施可能会抑制海马体中牛磺酸的释放。在所有组中，GABA和天冬氨酸水平在这2周内均未显示出显著变化。这些结果为解释康复过程中对缺血性损伤脑的神经化学影响提供了见解。
