Kpegba Kafui, Murtha Matthew, Nesnas Nasri
Department of Chemistry, Florida Institute of Technology, Melbourne, FL 32901, USA.
Bioorg Med Chem Lett. 2006 Mar 15;16(6):1523-6. doi: 10.1016/j.bmcl.2005.12.052. Epub 2005 Dec 27.
All trans retinal was attached to both the primary face and the secondary face of beta-cyclodextrin via a Schiff base linkage, analogous to that in rhodopsin. The new models were evaluated and compared with n-butylamine retinylidene Schiff base for their rates of hydrolysis, and factors that influence such rates. Competition studies using adamantane carboxylate demonstrated the kinetic trap theory by diminishing the binding of retinal in the cyclodextrin, thereby augmenting the rate of hydrolysis. NMR experiments indicate that the retinylidene is most probably bound in the form of a dimer.
全反式视黄醛通过席夫碱键连接到β-环糊精的主面和次面上，类似于视紫红质中的情况。对新模型进行了评估，并与正丁胺视黄亚基席夫碱的水解速率以及影响该速率的因素进行了比较。使用金刚烷羧酸盐的竞争研究通过减少视黄醛在环糊精中的结合，从而提高水解速率，证明了动力学陷阱理论。核磁共振实验表明，视黄亚基很可能以二聚体的形式结合。
