液相色谱-串联质谱法快速微量检测同型半胱氨酸
Rapid micro-scale assay for homocysteine by liquid chromatography-tandem mass spectrometry.
作者信息
Kuhn Joachim, Götting Christian, Kleesiek Knut
机构信息
Institut für Laboratoriums- und Transfusionsmedizin, Herz- und Diabeteszentrum Nordrhein-Westfalen, Universitätsklinik der Ruhr-Universität Bochum, Bad Oeynhausen, Germany.
出版信息
Clin Biochem. 2006 Feb;39(2):164-6. doi: 10.1016/j.clinbiochem.2005.11.009. Epub 2005 Dec 28.
OBJECTIVES
Increased total homocysteine (tHcy) level is an independent risk factor for atherosclerosis and cardiovascular disease. Here, we describe a rapid tHcy micro-scale assay.
METHODS
We developed an easy sample preparation and rapid liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the measurement of tHcy in human serum and plasma, respectively.
RESULTS
The tHcy assay was linear up to 61.6 micromol/L, the detection limit was 1.0 micromol/L, the lower limit of quantification was 1.8 micromol/L, the imprecision in the range of 9.30-25.9 micromol/L was less than 5.9% and the analytical recovery was 94.7 +/- 6.3%.
CONCLUSIONS
Our novel tHcy LC-MS/MS assay is a quick, precise and robust method for tHcy determination in routine diagnostics.
目的
总同型半胱氨酸（tHcy）水平升高是动脉粥样硬化和心血管疾病的独立危险因素。在此，我们描述一种快速的tHcy微量检测方法。
方法
我们分别开发了一种简便的样品制备方法和快速液相色谱-串联质谱（LC-MS/MS）方法，用于测定人血清和血浆中的tHcy。
结果
tHcy检测在高达61.6微摩尔/升时呈线性，检测限为1.0微摩尔/升，定量下限为1.8微摩尔/升，在9.30 - 25.9微摩尔/升范围内的不精密度小于5.9%，分析回收率为94.7±6.3%。
结论
我们新型的tHcy LC-MS/MS检测方法是一种用于常规诊断中tHcy测定的快速、精确且可靠的方法。
Zotero 插件