Casetta Bruno
Applied Biosystems, Monza, Italy.
Methods Mol Biol. 2010;603:253-60. doi: 10.1007/978-1-60761-459-3_23.
A detailed protocol using liquid chromatography coupled to tandem mass spectrometry (LC/MS/MS) is described for the determination of the total homocysteine (HCY) in plasma. Sample preparation is simple and relies on the complete reduction of any homocystine ((HCY)(2)) or protein-bound homocysteine, followed by the plasma protein precipitation. Any factor influencing the measurement is addressed. The protocol is robust and suitable for large-scale routine determinations.
本文描述了一种使用液相色谱-串联质谱联用（LC/MS/MS）测定血浆中总同型半胱氨酸（HCY）的详细方法。样品制备简单，依赖于将任何同型胱氨酸（(HCY)(2)）或与蛋白质结合的同型半胱氨酸完全还原，然后进行血浆蛋白沉淀。文中讨论了影响测量的任何因素。该方法稳健，适用于大规模常规测定。
