Kalinin Sergei V, Rodriguez B J, Shin J, Jesse S, Grichko V, Thundat T, Baddorf A P, Gruverman A
Condensed Matter Sciences Division, Oak Ridge National Laboratory, Oak Ridge, TN 37831, USA.
Ultramicroscopy. 2006 Mar;106(4-5):334-40. doi: 10.1016/j.ultramic.2005.10.005. Epub 2005 Dec 15.
Since the discovery in the late 18th century of electrically induced mechanical response in muscle tissue, coupling between electrical and mechanical phenomena has been shown to be a near-universal feature of biological systems. Here, we employ scanning probe microscopy (SPM) to measure the sub-Angstrom mechanical response of a biological system induced by an electric bias applied to a conductive SPM tip. Visualization of the spiral shape and orientation of protein fibrils with 5 nm spatial resolution in a human tooth and chitin molecular bundle orientation in a butterfly wing is demonstrated. In particular, the applicability of SPM-based techniques for the determination of molecular orientation is discussed.
自18世纪末发现肌肉组织中的电诱导机械响应以来，电现象和机械现象之间的耦合已被证明是生物系统几乎普遍存在的特征。在此，我们采用扫描探针显微镜（SPM）来测量施加到导电SPM尖端的电偏压所诱导的生物系统的亚埃机械响应。展示了在人类牙齿中以5纳米空间分辨率对蛋白质原纤维的螺旋形状和取向以及在蝴蝶翅膀中几丁质分子束取向的可视化。特别讨论了基于SPM的技术在确定分子取向方面的适用性。
