Lebedev Vladimir N, Kharovskaya Mariya I, Lazoryak Bogdan I, Solovieva Anastasiya O, Fadeeva Inna V, Amirov Abdulkarim A, Koliushenkov Maksim A, Orudzhev Farid F, Baryshnikova Oksana V, Yankova Viktoriya G, Rau Julietta V, Deyneko Dina V
Chemistry Department, Lomonosov Moscow State University, Leninskie Gory 1, 119991 Moscow, Russia.
Laboratory of Pharmacology Active Compounds, Research Institute of Clinical and Experimental Lymphology-Branch of the Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences (RICEL-Branch of IC&G SB RAS), 630060 Novosibirsk, Russia.
Biomimetics (Basel). 2024 Apr 20;9(4):252. doi: 10.3390/biomimetics9040252.
β-tricalcium phosphate (β-TCP) is a promising material in regenerative traumatology for the creation of bone implants. Previously, it was established that doping the structure with certain cations can reduce the growth of bacterial activity. Recently, much attention has been paid to co-doped β-TCP, that is explained by their ability, on the one hand, to reduce cytotoxicity for cells of the human organism, on the other hand, to achieve a successful antibacterial effect. Sr, Cu-co-doped solid solutions of the composition CaSrCu(PO) was obtained by the method of solid-phase reactions. The Rietveld method of structural refinement revealed the presence of Sr ions in four crystal sites: M1, M2, M3, and M4. The M5 site is completely occupied by Cu. Isomorphic substitution of Ca → (Srand Cu) expands the concentration limits of the existence of the solid solution with the β-TCP structure. No additional phases were formed up to = 4.5 in CaSrCu(PO). Biocompatibility tests were performed on cell lines of human bone marrow mesenchymal stromal cells (hMSC), human fibroblasts (MRC-5) and osteoblasts (U-2OS). It was demonstrated that cytotoxicity exhibited a concentration dependence, along with an increase in osteogenesis and cell proliferation. CaSrCu(PO) powders showed significant inhibitory activity against pathogenic strains Escherichia coli and Staphylococcus aureus. Piezoelectric properties of CaSrCu(PO) were investigated. Possible ways to achieve high piezoelectric response are discussed. The combination of bioactive properties of CaSrCu(PO) renders them multifunctional materials suitable for bone substitutes.
β-磷酸三钙（β-TCP）是再生创伤学中用于制造骨植入物的一种很有前景的材料。此前已证实，用某些阳离子掺杂该结构可以降低细菌活性的增长。最近，共掺杂的β-TCP受到了广泛关注，这一方面是因为它们能够降低对人体细胞的细胞毒性，另一方面是因为它们能够实现成功的抗菌效果。通过固相反应法获得了组成CaSrCu(PO)的Sr、Cu共掺杂固溶体。Rietveld结构精修方法揭示了Sr离子存在于四个晶体位点：M1、M2、M3和M4。M5位点完全被Cu占据。Ca→(Sr和Cu)的同构取代扩展了具有β-TCP结构的固溶体存在的浓度极限。在CaSrCu(PO)中，当 = 4.5时都没有形成额外的相。对人骨髓间充质基质细胞（hMSC）、人成纤维细胞（MRC-5）和成骨细胞（U-2OS）的细胞系进行了生物相容性测试。结果表明，细胞毒性呈现浓度依赖性，同时骨生成和细胞增殖增加。CaSrCu(PO)粉末对致病性大肠杆菌和金黄色葡萄球菌表现出显著的抑制活性。研究了CaSrCu(PO)的压电性能。讨论了实现高压电响应的可能途径。CaSrCu(PO)的生物活性特性使其成为适用于骨替代物的多功能材料。
