Sedlák K, Bártová E
Department of Virology and Serology, State Veterinary Institute Prague, Czech Republic.
Vet Parasitol. 2006 Mar 31;136(3-4):223-31. doi: 10.1016/j.vetpar.2005.11.021. Epub 2006 Jan 18.
Neospora caninum is an apicomplexan parasite that causes neuromuscular disease in dogs and abortions in cattle. Little is known about the prevalence of antibodies to this parasite in zoo animals. Sera from 556 animals, from 13 Czech and Slovak zoos were tested for antibodies to N. caninum and Toxoplasma gondii by indirect fluorescent antibody test. Antibodies to N. caninum were found in 31 of 556 zoo animals (5.6%), representing 18 of 114 species tested: Eurasian wolf (Canis lupus lupus), Maned wolf (Chrysocyon brachyurus), fennec (Vulpes zerda), cheetah (Acinonyx jubatus), jaguarundi (Herpailurus yaguarondi), Eurasian lynx (Lynx lynx), Indian lion (Panthera leo goojratensis), fisher (Martes pennanti), blackbuck (Antilope cervicapra), European bison (Bison bonasus), lechwe (Kobus leche), African buffalo (Syncerus caffer caffer), eland (Taurotragus oryx), sitatunga (Tragelaphus spekei gratus), Thorold's deer (Cervus albirostris), Eastern elk (C. elaphus canadensis), Vietnam sika deer (C. nippon pseudaxis) and Père David's deer (Elaphurus davidianus). Titres ranged from 1:40 to 1:2560. The highest prevalence 50% was found in family mustelidae of the order carnivora. Antibodies to T. gondii were detected in 193 of 556 zoo animals (34.7%) representing 72 of 114 species tested, with titres ranging from 1:40 to 1:40960. The highest prevalence 100% was found in families: hyaenidae, mustelidae, ursidae and viveridae of the order carnivora. The results of this study indicate that zoo animals have more exposure to T. gondii than to N. caninum. It is the first report of seroprevalence of antibodies to N. caninum in European zoo animals.
犬新孢子虫是一种顶复门寄生虫，可导致犬类神经肌肉疾病和牛流产。关于圈养动物中该寄生虫抗体的流行情况，人们了解甚少。通过间接荧光抗体试验，对来自捷克和斯洛伐克13家动物园的556只动物的血清进行了犬新孢子虫和刚地弓形虫抗体检测。在556只圈养动物中有31只（5.6%）检测到犬新孢子虫抗体，涉及所检测的114个物种中的18种：欧亚狼（Canis lupus lupus）、鬃狼（Chrysocyon brachyurus）、耳廓狐（Vulpes zerda）、猎豹（Acinonyx jubatus）、美洲豹猫（Herpailurus yaguarondi）、欧亚猞猁（Lynx lynx）、印度狮（Panthera leo goojratensis）、渔貂（Martes pennanti）、印度羚（Antilope cervicapra）、欧洲野牛（Bison bonasus）、水羚（Kobus leche）、非洲水牛（Syncerus caffer caffer）、大羚羊（Taurotragus oryx）、水薮羚（Tragelaphus spekei gratus）、白唇鹿（Cervus albirostris）、东加拿大马鹿（C. elaphus canadensis）、越南梅花鹿（C. nippon pseudaxis）和麋鹿（Elaphurus davidianus）。抗体滴度范围为1:40至1:2560。在食肉目鼬科动物中发现的最高流行率为50%。在556只圈养动物中有193只（34.7%）检测到刚地弓形虫抗体，涉及所检测的114个物种中的72种，抗体滴度范围为1:40至1:40960。在食肉目以下科中发现的最高流行率为100%：鬣狗科、鼬科、熊科和灵猫科。本研究结果表明，圈养动物接触刚地弓形虫的机会比犬新孢子虫更多。这是欧洲圈养动物中犬新孢子虫抗体血清流行率的首次报告。
