Bártová E, Sedlák K, Literák I
Department of Biology and Wildlife Diseases, University of Veterinary and Pharmaceutical Sciences, Palackého 1/3, 612 42 Brno, Czech Republic.
Vet Parasitol. 2006 Nov 30;142(1-2):150-3. doi: 10.1016/j.vetpar.2006.06.022. Epub 2006 Jul 31.
Sera collected from hunter-killed wild boars (Sus scrofa) during 1999-2005 from seven different regions of the Czech Republic were assayed for antibodies to Toxoplasma gondii by indirect fluorescence antibody test and to Neospora caninum by competitive-inhibition enzyme-linked immunosorbent assay and by indirect fluorescence antibody test. Antibodies to T. gondii were detected in 148 (26.2%) of 565 wild boars with serum dilutions of 1:40 in 40, 1:80 in 40, 1:160 in 27, 1:320 in 19, 1:640 in 18 and 1:1280 in 4 wild boars. Antibodies to N. caninum were detected in 102 (18.1%) of 565 wild boars with 30.1-94.6% inhibition in ELISA; statistical significant differences were observed between sampling regions, ranging from 0% to 31.8%. Sera, positive in ELISA, were examined in IFAT; 58 of 102 (56.9%) were positive with titres 1:40-1:160. Mixed infection (concurrent presence of both T. gondii and N. caninum antibodies) was found in 38 wild boars. It is the first report of antibodies to N. caninum in wild boar. Serological results indicate a common exposure to T. gondii and to N. caninum among wild boars in the Czech Republic.
对1999年至2005年间从捷克共和国七个不同地区猎捕的野猪（Sus scrofa）采集的血清，通过间接荧光抗体试验检测弓形虫抗体，通过竞争抑制酶联免疫吸附试验和间接荧光抗体试验检测犬新孢子虫抗体。在565头野猪中，148头（26.2%）检测到弓形虫抗体，其中血清稀释度为1:40的有40头，1:80的有40头，1:160的有27头，1:320的有19头，1:640的有18头，1:1280的有4头。在565头野猪中，102头（18.1%）检测到犬新孢子虫抗体，酶联免疫吸附试验抑制率为30.1%至94.6%；各采样地区之间观察到统计学上的显著差异，范围为0%至31.8%。对酶联免疫吸附试验呈阳性的血清进行间接荧光抗体试验检测；102份血清中有58份（56.9%）呈阳性，滴度为1:40至1:160。在38头野猪中发现混合感染（同时存在弓形虫和犬新孢子虫抗体）。这是首次在野猪中报告犬新孢子虫抗体。血清学结果表明，捷克共和国的野猪普遍接触弓形虫和犬新孢子虫。
