College of Veterinary Medicine, Yangzhou University, 12 East Wenhui Road, Yangzhou, Jiangsu, 225009, People's Republic of China.
Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou, 225009, China.
BMC Vet Res. 2022 Jun 3;18(1):213. doi: 10.1186/s12917-022-03308-2.
Eimeria coccidiosis is a significant intestinal parasitic disease, which can lead to weight loss, disease and even death of many animals. At present, there is no information about the prevalence of Eimeria among the world's endangered species of Père David's deer (Elaphurus davidianus). Therefore, the purpose of this study is to identify an unknown Eimeria genus in the Père David's deer in Dafeng Milu National Nature Reserve, China.
A new Eimeria species is described from Père David's deer. Sporulated oocysts (n = 54) are pyriform, with a rough, yellowish brown, 2-layered oocyst wall (2.5 μm thick). A numerous small granules are dispersed randomly on the wall. Oocysts measured 41.2 (39.2-42.8) μm × 29.5 (27.9-30.5) μm, oocyst length/width (L/W) ratio, 1.4. Oocyst residuum, a polar granule and a polar cap are absent. The micropyle (3.5 μm wide) is present. Sporocysts are spindle shaped, 18.2 (16.5-20.0) μm × 10.5 (9.8-11.9) μm, sporocyst L/W ratio, 1.7 (1.5-1.9). A thin convex Stieda body is present and the sporocyst residuum is composed of numerous small granules less than 2.0 μm in diameter dispersed randomly. Each sporocyst contained 2 comma-shaped sporozoites in head-to-tail arrangement. A nucleus is located immediately anterior to the posterior, strong refractive and subspherical refractile body (~ 8 μm). Molecular analysis was conducted at the 18S, ITS-1 and COI loci.
Based on the morphological and molecular data, this isolate is a new species of coccidian parasite, which is named Eimeria davidianusi after its host, the Père David's deer (Elaphurus davidianus).
艾美耳球虫病是一种严重的肠道寄生虫病，可导致许多动物体重减轻、患病甚至死亡。目前，关于世界濒危物种麋鹿（Elaphurus davidianus）中艾美耳球虫的流行情况尚无信息。因此，本研究的目的是在中国大丰麋鹿国家级自然保护区鉴定麋鹿中的一种未知艾美耳球虫属。
从麋鹿中描述了一种新的艾美耳球虫种。孢子化卵囊（n=54）呈梨形，具有粗糙的黄棕色 2 层卵囊壁（厚 2.5μm）。壁上散布着许多小颗粒。卵囊大小为 41.2（39.2-42.8）μm×29.5（27.9-30.5）μm，卵囊长/宽（L/W）比为 1.4。无卵囊残体、极粒和极帽。有一个 3.5μm 宽的微孔。孢子囊呈纺锤形，18.2（16.5-20.0）μm×10.5（9.8-11.9）μm，孢子囊 L/W 比为 1.7（1.5-1.9）。有一个薄的凸斯氏位体，孢子囊残体由许多直径小于 2.0μm 的小颗粒随机散布组成。每个孢子囊内含 2 个以头对头方式排列的逗号形孢子虫。一个核位于后极之前，强折射和近球形折射体（~8μm）。在 18S、ITS-1 和 COI 基因座进行了分子分析。
根据形态学和分子数据，该分离株是一种新的球虫寄生虫种，以其宿主麋鹿（Elaphurus davidianus）命名为麋鹿艾美耳球虫（Eimeria davidianusi）。
